1. Microscopes

2. Some Microscope Parts

3. (lens to ‘see’ through; Magnifies 10x) (Carry microscope) (lenses close to object/specimen; Magnifies 10x, 20x, or 40x) NOSE PIECE (select objective lens) STAGE (holds slide) (Lifts or lowers stage to produce a focused image) (focuses/condenses light on specimen) (a.k.a body tube/keeps eyepiece at correct distance from objective) (sharpens focus)

4. Iris Diaphragm (open 1/close 5) Can be used to increase contrast (close 5) Or increase amount of light (open 1) Diaphragm Stage Clips Diaphragm lever

5. Magnifying Power Eyepiece x objective lens = TOTAL magnification –LOW POWER: 10x X 10x = 100x –HIGH POWER: 10x X 43x = 430x correct units for a microscope = micrometer (µm) 1 µm = (1/1000) mm

6. KEEP IT CLEAN! When to use… Objective lens Glass slides Cover slips CondenserEye piece Lens tissue Slide tissue X X X XX

7. Sequence for Viewing 1.plug in & turn on 2.find object in LOW POWER 3.focus with coarse adjustment & adjust light (light: 1 lets in the most & 5 least) 4.center object (still in low power) 5.turn to high power objective without changing focus 6.re-adjust light 7.sharpen focus with fine adjustment

8. PARFOCAL ability for the microscope to remain in focus when objective lens is changed Low power High Power

9. Make a “Wet Mount” Cover slip Lower Slide 1 2

10. Store your Microscope Properly 1.Take slide OFF 2.Put lower power above stage 3.Roll nosepiece up (old); Stage down (new) 4.Turn OFF light 5.Wrap cord around scope

11. How not to break the microscope Always carry the microscope with 2 hands, one hand under the base & the other grasping the arm.

12. Discussion and Summary H A F Appears on stage H AF Appears in the Eyepiece

13. It _____________________ It turns it _______________ & _____________________ What a microscope does to an image… Magnifies Upside down Backwards

14. As the power of the microscope Magnification increases –The field of vision –The depth of field –Amount of light passing through the lens system

15. 0.2  m 0.1nm