1. Stomata An Inquiry Approach

2. Background

3. 3 Stomata http://www.youtube.com/watch?v=cFX4JrsPaUs http://www.youtube.com/watch?v=cFX4JrsPaUs

4. Skills

5. 5 How to: Microscopic Measurement Determine the diameter of your “field of view.” 1. Begin with a clear ruler. 2. Place on the stage, visible under the lowest magnification for your microscope. 3. Determine the diameter of the field of view in mm. 4. 1mm=1000 Micrometers (μm) 1 mm

6. How to: FOV under a higher power lens For a higher power, you need to calculate the field of view.

7. 7 Zoom in (Original Obj/New Obj) x Original FOV=New FOV FOV=Field of View 7 Original Objective New Objective Original FOV (μm) New FOV 4x10x45001800 4x40x4500450 4x100x4500180

8. 8 Stomata Prints Paint a small amount of polish on a small area of leaf Let dry Lay tape on polish and press firmly Peel off, tape on slide. Clear Fingernail Polish Clear tape Glass Slide

9. Stomata Questions

10. Onion Osmosis Analysis with Image J

11. Cut a piece of outer pigmented tissue

13. Make a wet mount

14. Add concentrated salt solution

15. Take a picture through the microscope. Save to computer.

16. Determining Percent Change in Area Find your file and open it.

17. To get area and perimeter... Select the straight line tool. Drag across your field of view.

18. Under analyze: Set scale

19. Pixels are already there. Enter your known distance.

20. Analyze: Set Measurements

21. Trace with the freehand tool Go to analyze: Measure Compare before and after