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Next Generation Sequencing. Overview of RNA-seq experimental procedures. Wang L et al. Briefings in Functional Genomics 2010;9:118-128 © The Author.

Published byOlivia Cole Modified over 9 years ago

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Presentation on theme: "Next Generation Sequencing. Overview of RNA-seq experimental procedures. Wang L et al. Briefings in Functional Genomics 2010;9:118-128 © The Author."— Presentation transcript:

1 Next Generation Sequencing

2

3 Overview of RNA-seq experimental procedures. Wang L et al. Briefings in Functional Genomics 2010;9:118-128 © The Author 2010. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

4 FASTQ format Sequence Quality score encoded in ASCII to save space Machine ID SRA ID & pair info Flowcell laneFlowcell coordinates

5 Sequence reads aligned using TopHat

6 Relative expression of transcript (determined by CuffLinks) represented as RPKMs or FPKMs RPKM: Reads Per Kilobase of transcript per Million mapped reads. FPKM: Fragments Per Kilobase of transcript per Million mapped reads.

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