1. Quiz

2. Bacterial strain Minimal medium glucose 25 o Minimal medium glucose 40 0 Minimal medium glucose and 0.4M mannitol 25 o Minimal medium glucose and 0.4M mannitol 40 o Wild type TS mutant which cannot make peptidoglycan at 40 o TS mutant which cannot initiate DNA replication at 40 o TS mutant which cannot make the O- antigen at 40 o Bacteria treated with chloramphenicol which freezes the ribosome in place XXXXXXX XXXXX XXXXXXXX XXXXX Bacteria treated with cyclohexamide which inhibits 80S ribosomes XXXXXXX XXXXX XXXXXXXX XXXXX TS mutant which cannot make flagella XXXXXXX XXXXX XXXXXXXX XXXXX

3. Bacterial strain Minimal medium glucose 25 o Minimal medium glucose 40 0 Minimal medium glucose and 0.4M mannitol 25 o Minimal medium glucose and 0.4M mannitol 40 o Wild type + + + + TS mutant which cannot make peptidoglycan at 40 o + die ++ TS mutant which cannot initiate DNA replication at 40 o + - alive + TS mutant which cannot make the O- antigen at 40 o + +++ Bacteria treated with chloramphenicol which freezes the ribosome in place - alive XXXXXXX XXXXX XXXXXXXX XXXXX Bacteria treated with cyclohexamide which inhibits 80S ribosomes ++ XXXXXXX XXXXX XXXXXXXX XXXXX TS mutant which cannot make flagella ++XXXXXXX XXXXX XXXXXXXX XXXXX