1. A foam noodle model of restriction enzyme digestions Stephen Flanagan Purvis High School Research based lesson 1 class, 18 students 10.27.2014

2. Objectives BIO1: Examine the life processes to conclude the role enzymes play in regulating biochemical reactions. (DOK 2) Examine inheritance patterns using current technology (e.g., pedigrees, karyotypes, gel electrophoresis). (DOK 2) Genetics: Distinguish and explain the applications of various tools and techniques used in DNA manipulation (DOK 1)

3. Recently, I have been digesting genes using restriction enzymes I am looking at the speciation event between these 2 species, which requires a means to accurately differentiate species The haplotypes I find in these 2 fish species are only associated with only a single species

4. AGCTAAATTGGCTTCT–3’ AGCTAAATTGCCTTCT-3’

5. Engage the how many “species” game

6. Explore

7. Explain

8. Elaborate toothpick the fragments back together and look for more restriction sites Alu1 then compare with everyone else in the class and see if this new cut pattern would be able to differentiate the 2 species

9. Evaluate Built into the handout was a series of questions about how / what they were doing

10. http://4.bp.blogspot.com/

11. lesson assessment I would do this for a genetics course concept was hard for them to grasp the overall importance of telling one species from another was not clear to them http://kfcusa.org/storage/hands-raised.jpg