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A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah.

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Presentation on theme: "A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah."— Presentation transcript:

1 A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah Tavor-Deslex, Dmytro Lituiev, Katalin Enkerli, Paul T. Tarr, and Bruno Müller Plantphysiol Volume 161(3):1066-1075 February 28, 2013 ©2013 by American Society of Plant Biologists

2 Optimization of TCS. A, A concatemer of 24 repeats of the 5′-(A/G)GAT(C/T)T-3′ binding motif caused the strongest cytokinin-dependent induction of a LUC reporter in transient transfection assays. Zürcher E et al. Plantphysiol 2013;161:1066-1075 ©2013 by American Society of Plant Biologists

3 Sensitivity and specificity of TCSn::LUC in transient transfection assays. Zürcher E et al. Plantphysiol 2013;161:1066-1075 ©2013 by American Society of Plant Biologists

4 TCSn::GFP in the seedling. Zürcher E et al. Plantphysiol 2013;161:1066-1075 ©2013 by American Society of Plant Biologists

5 TCSn::GFP expression in different developmental contexts. Zürcher E et al. Plantphysiol 2013;161:1066-1075 ©2013 by American Society of Plant Biologists


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