1. Topic 7: Cellular Genetics. Exercise II Mitosis Cell Cycle Exp: Cell cycle onion tips Topic: DNA Exercise I: Isolation Analysis Plasmid DNA

2. Onion root tip.

5. Structure of bacteria.

6. Two strains of bacteria: pBR322 and pUC18

7. Isolation of Plasmid DNA: 1. Collect bacteria.

8. Isolation of plasmid DNA: 2. Add:

9. Isolation of Plasmid DNA: 3. Precipitate proteins, chromosomal DNA, and some of the RNA.

10. Isolation of plasmid DNA: Note: Addition of very small quantities of liquids.

11. Isolation of plasmid DNA: 4. DNAse and RNAse treatments.

12. Agarose Gel.

14. Electrophoresis:

16. Isolation of Plasmid DNA.

17. pBR322 pUC18 ET D R

18. pUC18pBR322 ET D R ET R D