1. POPULATION GENETICS 1

2. Outcomes 4. Discuss the application of population genetics to the study of evolution. 4.1 Describe the concepts of the deme and the gene pool. 4.2 Consider the Hardy-Weinberg principle. 4.3 Describe the factors which influence genetic drift. 4.4 Consider the relevance of the gene pool and the idea of mutations to the concept of evolution which will be studied later in unit 5. 2

3. The Gene Pool Members of a species can interbreed & produce fertile offspring Species have a shared gene pool Gene pool – all of the alleles of all individuals in a population 3

4. The Gene Pool Different species do NOT exchange genes by interbreeding Different species that interbreed often produce sterile or less viable offspring e.g. Mule 4

5. Populations A group of the same species living in an area No two individuals are exactly alike (variations) More Fit individuals survive & pass on their traits 5

6. Speciation Formation of new species One species may split into 2 or more species A species may evolve into a new species Requires very long periods of time 6

7. MODERN EVOLUTIONARY THOUGHT

8. Modern Synthesis Theory Combines Darwinian selection and Mendelian inheritance Population genetics - study of genetic variation within a population Emphasis on quantitative characters (height, size …) 8

9. Modern Synthesis Theory 1940s – comprehensive theory of evolution (Modern Synthesis Theory) Introduced by Fisher & Wright Until then, many did not accept that Darwin’s theory of natural selection could drive evolution 9 S. Wright A. Fisher

10. Modern Synthesis Theory TODAY’S theory on evolution Recognizes that GENES are responsible for the inheritance of characteristics Recognizes that POPULATIONS, not individuals, evolve due to natural selection & genetic drift Recognizes that SPECIATION usually is due to the gradual accumulation of small genetic changes 10

11. Microevolution Changes occur in gene pools due to mutation, natural selection, genetic drift, etc. Gene pool changes cause more VARIATION in individuals in the population This process is called MICROEVOLUTION Example: Bacteria becoming unaffected by antibiotics (resistant) 11

12. HARDY-WEINBERG PRINCIPLE

13. The Hardy-Weinberg Principle Used to describe a non-evolving population. Shuffling of alleles by meiosis and random fertilization have no effect on the overall gene pool. Natural populations are NOT expected to actually be in Hardy- Weinberg equilibrium. 13

14. The Hardy-Weinberg Principle Deviation from Hardy-Weinberg equilibrium usually results in evolution Understanding a non-evolving population, helps us to understand how evolution occurs 14.

15. 5 Assumptions of the H-W Principle 1.Large population size - small populations have fluctuations in allele frequencies (e.g., fire, storm). 2.No migration - immigrants can change the frequency of an allele by bringing in new alleles to a population. 3.No net mutations - if alleles change from one to another, this will change the frequency of those alleles 15

16. 5 Assumptions of the H-W Principle 3.Random mating - if certain traits are more desirable, then individuals with those traits will be selected and this will not allow for random mixing of alleles. 4.No natural selection - if some individuals survive and reproduce at a higher rate than others, then their offspring will carry those genes and the frequency will change for the next generation. 16

17. Traits Selected for Random Mating 17

18. The Hardy-Weinberg Principle The gene pool of a NON-EVOLVING population remains CONSTANT over multiple generations (allele frequency doesn’t change) The Hardy-Weinberg Equation: 1.0 = p 2 + 2pq + q 2 Where: p 2 = frequency of AA genotype 2pq = frequency of Aa q 2 = frequency of aa genotype 18

19. The Hardy-Weinberg Principle Determining the Allele Frequency using Hardy-Weinberg: 1.0 = p + q Where: p = frequency of A allele q = frequency of a allele 19

20. 20 Allele Frequencies Define Gene Pools As there are 1000 copies of the genes for color, the allele frequencies are (in both males and females): 320 x 2 (RR) + 160 x 1 (Rr) = 800 R; 800/1000 = 0.8 (80%) R 160 x 1 (Rr) + 20 x 2 (rr) = 200 r; 200/1000 = 0.2 (20%) r 500 flowering plants 480 red flowers20 white flowers 320 RR160 Rr20 rr

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23. MICROEVOLUTION OF SPECIES

24. Causes of Microevolution Genetic Drift - the change in the gene pool of a small population due to chance Natural Selection - success in reproduction based on heritable traits results in selected alleles being passed to relatively more offspring (Darwinian inheritance) - Cause ADAPTATION of Populations Gene Flow -is genetic exchange due to the migration of fertile individuals or gametes between populations 24

25. Causes of Microevolution Mutation - a change in an organism’s DNA - Mutations can be transmitted in gametes to offspring Non-random mating - Mates are chosen on the basis of the best traits 25

26. GENETIC DRIFT

27. Factors that Cause Genetic Drift Bottleneck Effect - a drastic reduction in population (volcanoes, earthquakes, landslides …) - Reduced genetic variation - Smaller population may not be able to adapt to new selection pressures Founder Effect - occurs when a new colony is started by a few members of the original population - Reduced genetic variation - May lead to speciation 27

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29. Loss of Genetic Variation Cheetahs have little genetic variation in their gene pool This can probably be attributed to a population bottleneck they experienced around 10,000 years ago, barely avoiding extinction at the end of the last ice age 29

31. Founder’s Effect 31

32. MODES OF NATURAL SELECTION

33. Modes of Natural Selection Directional Selection - Favors individuals at one end of the phenotypic range - Most common during times of environmental change or when moving to new habitats Disruptive selection - Favors extreme over intermediate phenotypes - Occurs when environmental change favors an extreme phenotype 33

34. 34 Directional Selection

35. Disruptive Selection 35

36. Modes of Natural Selection Stabilizing Selection - Favors intermediate over extreme phenotypes - Reduces variation and maintains the cureent average - Example: Human birth weight 36

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38. VARIATIONS IN POPULATIONS

39. Geographic Variations Variation in a species due to climate or another geographical condition Populations live in different locations Example: Finches of Galapagos Islands & South America 39

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41. Heterozygote Advantage Favors heterozygotes (Aa) Maintains both alleles (A,a) instead of removing less successful alleles from a population Sickle cell anemia > Homozygotes exhibit severe anemia, have abnormal blood cell shape, and usually die before reproductive age. > Heterozygotes are less susceptible to malaria 41

42. Sickle Cell and Malaria 42

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44. Other Sources of Variation Mutations - In stable environments, mutations often result in little or no benefit to an organism, or are often harmful - Mutations are more beneficial (rare) in changing environments (Example: HIV resistance to antiviral drugs) Genetic Recombination - source of most genetic differences between individuals in a population Co-evolution -Often occurs between parasite & host and flowers & their pollinators 44