1. Pharos university Faculty of Allied Medical SCIENCE Clinical Laboratory Instrumentation (MELI-201) Dr. Tarek El Sewedy

2. Mid Term Revision

3.  The WHO has classified clinical laboratories according to their ……………….. Into ……….. Classes.  ……………………….. is an example of Risk group I as classified by WHO  ……………………………………….. Laboratories should be located in separate buildings and strictly access controlled.  …………………….. is a type of centrifuge that is used for the isolation of cellular macromolecules, ribosomes due to its high speed.  The basic components of a centrifuge are; shaft, bucket and ……………… which is the part that holds the bucket.  It is advisable to add a ……………………….. agent to the water bath To minimize the growth of ………………….in the water.  Never forget to ……………………….. the tubes before starting to spin any centrifuge or they might break or damage the centrifuge.

4.  The presence of a decontaminating autoclave is not essential WHO biosafety requirement in laboratory level (1 – 2 -3 -4 -all)  In centrifugation (smaller -heavier-bigger-lighter-sharper) particles sediment first.  It is advisable to store acids in (plastic-polystyrene-glass-nylon- polypropylene) containers.  Ultracentrifuge tubes should be balanced by (weight-volume-sight- thickness-rotor).  Indirect ELISA is used to measure (antibodies-Antigens-None)

5.  Mentions 3 different applications of Flowcytometers in medicine.  Mention the main advantages and disadvantages of Heamocytometer  Mention the criteria for Sorting of cells using a flowcytometer.

6.  Mentions 3 different applications of PCR  Mention the main difference between different types of incubators

7.  Mention the main advantages and disadvantages of Heamocytometer

8.  Mentions 3 different applications of Electrophoresis  Mention the main difference between DNA and Protein electrophoresis

9. Suggesting reading  Encyclopedia of Medical Devices and Instrumentation, 2nd ed. New York: Wiley, 2006