1. AP Biology Biotechnology Part 2 Genetics of Bacteria

2. Bacterial “size”

3. Prokaryote Structure

4. Bacterial genome

5. Theta Replication Origin of replication Replication fork Termination of replication

6. Transformation

7. Transduction A+A+ Phage DNA A+A+ Donor cell B+B+ A+A+ B+B+ Crossing over A+A+ A–A– B–B– Recipient cell A+A+ B–B– Recombinant cell

8. . Sex pilus 5 µm

9. DNA transfer “Conjugation”

10. Barbara McClintock Maize

11. Transposon -Composite 5 3 3 5 Transposon Insertion sequence Insertion sequence Antibiotic resistance gene Transposase gene Inverted repeat

12. Transposons

13. Operon Regulation Promoter DNA trpR Regulatory gene RNA polymerase mRNA 3 5 Protein Inactive repressor Tryptophan absent, repressor inactive, operon on mRNA 5 trpE trpD trpC trpBtrpA Operator Start codon Stop codon trp operon Genes of operon E Polypeptides that make up enzymes for tryptophan synthesis D C B A

14. Repressor Operon

15. Operon Repressor DNA Protein Tryptophan (corepressor) Tryptophan present, repressor active, operon off mRNA Active repressor No RNA made

16. Inducer Operon