1. AP Biology Discussion Notes Friday 3/6/2015

2. Goals for Today Be able to describe the difference in the 2 +pGLO plates using our Operon Vocabulary Be able to describe what it means and why genes from a jellyfish can function in bacteria. Be able to analyze transformation efficiency and describe what that calculation actually means.

3. Question of the day 3/06 What kind of Operon is normally in the “on” position, how is this accomplished, and what type of control would this exhibit? Give an example of this type of operon. Is this the operon in our lab

4. Desired Lab Results

5. Lab Analysis Be able to explain the predictions/results discussing OPERONS!

6. What is this thing we inserted?

7. Arabinose Operon

8. GFP gene

9. Arabinose Operon OPERON VOCABULARY to use in your lab explanation. Describe what is going on in the 2 +pGLO plates using the terms: –Inducible/repressible –Positive/negative control –Promoter –Operator –Repressor Protein –Transcription GFP gene

10. Lab Analysis Be able to explain the predictions/results discussing OPERONS! 1.Work through lab analysis & calculations together. (Yellow sheet) and ASK QUESTIONS! 2.Next answer white sheet as a group.

11. What is transformation efficiency? What is the unit? Is this number bigger or smaller than your number of colonies? Did we add a whole µg?

12. Questions on the Lab? LAB QUIZ on TUESDAY