1. RapidVet ® -H Blood Group Determination System for Identifying DEA 1.1 Positive and DEA 1.1 Negative Dogs

2. Kit contains: auto-agglutination saline screen cards, patient test cards, pipettes, stirrers, diluent, instructions, interpretation guide and report card. Shelf-life: 24 months at room temperature

3. If agglutination is observed, STOP the test and perform cell washing procedures before proceeding. If auto-agglutination does not occur, proceed to the next step. Prior to typing, check for auto-agglutination using the card provided in the kit. This card is used for three patients and, after use, should be returned to the kit box.

4. Remove the Test Card from its plastic sleeve. Write the name/number of the dog and the testing date on the card.

5. Dispense 1 drop of diluent from the dropping bottle into each well.

6. Aspirate a small amount of patient sample into a pipette and release 1 drop (50µl) into each well, taking care not to cross-contaminate the wells.

7. Using a NEW stirrer and pressing downward with the flat portion of the stirrer, spread and mix the materials within the entirety of the first well for about 10 seconds.

8. Using a NEW stirrer, spread and mix the materials within the entirety of the second well for about 10 seconds.

9. Using a NEW stirrer, spread and mix the materials within the entirety of the third well for about 10 seconds.

10. Rock the card, using a transaxial motion, for 1 minute. Be sure materials are mixing and “rotating” within each individual well. Be careful not to cross-contaminate.

11. Read the results, noting whether agglutination has or has not occurred in the “Patient Test” well. DEA 1.1+ DEA 1.1 

12. After the card has been read, take a digital photograph for a permanent record, or allow to dry and replace in its plastic sleeve as a permanent record.