Presentation is loading. Please wait.

Presentation is loading. Please wait.

Direct calibration of microarray probes Making a DNA-meter Alex Pozhitkov, Peter A. Noble, Diethard Tautz, Ploen Workshop, 2011.

Published byAiden MacKay Modified over 11 years ago

Similar presentations

Presentation on theme: "Direct calibration of microarray probes Making a DNA-meter Alex Pozhitkov, Peter A. Noble, Diethard Tautz, Ploen Workshop, 2011."— Presentation transcript:

1 Direct calibration of microarray probes Making a DNA-meter Alex Pozhitkov, Peter A. Noble, Diethard Tautz, Ploen Workshop, 2011.

2 Conundrum Signal intensity vs. GC Signal intensity vs. G 0 Sense vs. antisense (same genomic loci) R 2 =0.37 Confirms: Pozhitkov, et al (2006). Tests of rRNA hybridization … cannot be predicted. NAR 34, e66-e6

3 Melting study Overhangs Buffer composition Tm Sense vs. Tm antisense Tm vs. Signal Intensity on the array

4 Melting Temperatures Tm Sense ( 0 C) Tm Antisense ( 0 C)

5 Microarray is an Instrument Instruments require calibration

6 Good, Bad and Noise Individual replicasAverages 800 600 400 200 0 2 4 6 8 5000 4000 3000 2000 1000 0 2 4 6 8 Concentration Signal Intensity

7 Freundlich isotherm! y=ax b Langmuir isotherm is not the only possible isotherm: Pozhitkov et al. (2010) Beyond Affymetrix arrays: … NAR 38, e28.

8 DNA and RNA y=ax b +c

9 Measurements and Errors Single probe replicateAverage over 10 replicates

10 CNV detection CNV expected: 1

11 Sample prep variability loci $

12 Acknowledgements Max Planck Society Alabama State University

Download ppt "Direct calibration of microarray probes Making a DNA-meter Alex Pozhitkov, Peter A. Noble, Diethard Tautz, Ploen Workshop, 2011."

Similar presentations

$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300.

$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300.
$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300.

$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300.
$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200.

$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200.
$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300.

$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300.
$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500.

$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500.
Power of Four LiveWell Consulting. What does it take? Open 3 Business Centers Commit to 200 point autoship Enroll 4 people in 1st 8 weeks Platinum Pace.

Power of Four LiveWell Consulting. What does it take? Open 3 Business Centers Commit to 200 point autoship Enroll 4 people in 1st 8 weeks Platinum Pace.
Chapter 7 Algebra II Review JEOPARDY Jeopardy Review.

Chapter 7 Algebra II Review JEOPARDY Jeopardy Review.
$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300.

$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300.
Detecting Degradation in DNA samples

Detecting Degradation in DNA samples
Figure S1: Clustering analysis of the expression ratio of the sense and antisense pair of transcripts A B C D E F A B C D E F dT Random Sense : Antisense.

Figure S1: Clustering analysis of the expression ratio of the sense and antisense pair of transcripts A B C D E F A B C D E F dT Random Sense : Antisense.
Revenue and Profit.

Revenue and Profit.
Analysis of Affymetrix expression data using R on Azure Cloud Anne Owen Department of Mathematical Sciences University of Essex 15/16 March, 2012 SAICG.

Analysis of Affymetrix expression data using R on Azure Cloud Anne Owen Department of Mathematical Sciences University of Essex 15/16 March, 2012 SAICG.
Instructions for Playing Jeopardy Click on the question that you want to attempt, example $100 Read the question and click on the slide to advance to.

Instructions for Playing Jeopardy Click on the question that you want to attempt, example $100 Read the question and click on the slide to advance to.
Who Wants To Be A Millionaire? Mrs Mances Edition.

Who Wants To Be A Millionaire? Mrs Mances Edition.
Can you explain the ideas

Can you explain the ideas
1 Water vapour self-continuum in windows: Processing the recent MSF RAL measurements Igor Ptashnik University of Reading CAVIAR semi-annual meeting 29.09.2010.

1 Water vapour self-continuum in windows: Processing the recent MSF RAL measurements Igor Ptashnik University of Reading CAVIAR semi-annual meeting
1 Water vapour continuum in windows: Processing the recent MSF RAL measurements Igor Ptashnik Dept. of Meteorology, University of Reading 10 February 2011.

1 Water vapour continuum in windows: Processing the recent MSF RAL measurements Igor Ptashnik Dept. of Meteorology, University of Reading 10 February 2011.

Real-Time Gas Lift Optimization

Real-Time Gas Lift Optimization
Category 1 100 200 300 400 500 Category 2Category 3Category 4Category 5 100.

Category Category 2Category 3Category 4Category

Similar presentations

Ads by Google