1. New antiplasmodial alkaloids from Stephania rotunda Lour. () Sothavireak BORY 1, Sok Siya BUN 2, Béatrice BAGHDIKIAN 2, Valérie MAHIOU 2, Nadine AZAS 2, Hot BUN 2, Evelyne OLLIVIER 2. 1 Faculty of Pharmacy, University of Health Sciences-Cambodia. 2 UMR-MD3, Faculty of Pharmacy, Aix-Marseille University, France Symposium on Biodiversity and Health 17-18 November 2014

2. INTRODUCTION 2 30 medicinal plants suspected of antiplasmodial activity Symposium on Biodiversity and Health 17-18 November 2014

3. INTRODUCTION 3 Stephania rotunda Lour. Extract of tuber: IC 50 < 5 µg/mL Extract of tuber: IC 50 < 5 µg/mL Stephania rotunda presents the most interesting antiplasmodial activity. Symposium on Biodiversity and Health 17-18 November 2014

4. OBJECTIVES  Isolation and identification of the unknown alkaloids observed on HPLC chromatograms for the different extracts,  Evaluation of the antiplasmodial activity of alkaloids from the extracts of Stephania rotunda. 4 Symposium on Biodiversity and Health 17-18 November 2014

5. MATERIALS AND METHODS 5  Plant material  Intrumentation HPLC FC MS NMR Phytochemistry: Antiplasmodial activity: W 2 Strain of Plasmodium falciparum - IC 50 - SI - IC 50 - SI Symposium on Biodiversity and Health 17-18 November 2014

6. RESULTS & DISCUSSION 6  Phytochimical study: HPLC, MS, 1D and 2D NMR Cepharanthine Xylopinine Tetrahydropalmatine Roemerine Pseudopalmatine Palmatine Symposium on Biodiversity and Health 17-18 November 2014

7. RESULTS & DISCUSSION 7  Phytochimical study: HPLC, MS, 1D and 2D NMR Stephanine Vireakine Symposium on Biodiversity and Health 17-18 November 2014

8. RESULTS & DISCUSSION  In vitro antiplasmodial activity 8 Symposium on Biodiversity and Health 17-18 November 2014

9. RESULTS & DISCUSSION 9 Symposium on Biodiversity and Health 17-18 November 2014 CXTPCHLART CAdditive SynergismAdditive X Synergism TAdditiveSynergieAdditive P Antagonism Additive CHLSynergieAdditive Antagonism ARTAdditive X : xylopinine, C : cepharanthine, T : tetrahydropalmatine, P: palmatine, CHL: chloroquine, ART: artesunate.  In vitro antiplasmodial activity: Isobologram study

10. CONCLUSION 10  8 major alkaloids have been isolated and identified,  Vireakine  Vireakine is identified as a new compound,  Pseudopalmatinestephaninevireakine  Pseudopalmatine, stephanine and vireakine are described for the first time in the plant.  Phytochemical study:  Pseudopalmatine, cepharanthine, stephanine and vireakine are the most active compounds,  Isobologram: 2 synergistic effects and 1 antagonistic effect,  In vitro antiplasmodial activity: This results justify the use of Stephania rotunda as traditional medicine for the treatment of malaria. Select the most promising drug-candidates for further investigations. Symposium on Biodiversity and Health 17-18 November 2014

11. 11 2010 2012 2013 2014 2010 Symposium on Biodiversity and Health 17-18 November 2014

12. 12 THANK YOU FOR YOUR ATTENTION Symposium on Biodiversity and Health 17-18 November 2014