1. Cells

2. By the end of this class you should understand: The three major parts of all cells The primary structures found in prokaryotic and eukaryotic cells The major organelles found in all living things The basic functions of membrane-bound organelles found in eukaryotes

3. Parts of a Cell There are two major types of cells – Prokaryotic cell – Eukaryotic cell All cells have three parts: – Cell membrane – Genetic information – Cytoplasm

4. Prokaryotic Cells Prokaryotic cells are cells without a nucleus – All genetic info is bundled but not separated from cytoplasm All known prokaryotes are single-celled bacteria – Prokaryotic cells are tiny!

5. Eukaryotic Cells If every cell was a company: – Prokaryotic cells are a 1- man startup in a garage – Eukaryotic cells are a large corporation Eukaryotic cells have organelles which each perform separate jobs Genetic info stored in nucleus

6. Eukaryotes Some eukaryotes are single celled organisms – One eukaryotic cell performs all functions of life Some eukaryotes are multicellular – The different cells specialize in different tasks – Each cell is still individually alive!

7. Key Parts of Eukaryotic Cells Nucleus – Stores genetic information Cytoplasm – Composed of a liquid called cytosol – Filled with various membrane- bound organelles Cell Membrane – Often referred to as plasma membrane

8. Key Parts of Prokaryotic Cells Cell membrane, nucleic acids, and cytoplasm – Same as eukaryotes Cytoplasm is much simpler – Prokaryotes have NO membrane-bound organelles – They do have a cytoskeleton and ribosomes!

9. Cell Membrane The cell membrane is a flexible, selectively permeable (or semipermeable) barrier Boundary of cell, visible under microscope only as border of cell NOT the same as a cell wall – Cell wall is rigid

10. Cell Membrane Structure The cell membrane is composed of phospholipids – Hydrophilic head, hydrophobic tail The nonpolar tails orient themselves into a hydrophobic zone surrounding the cell – Essentially a thin bubble of oil

11. Cell Membrane Structure

12. Cell Membrane Proteins Proteins are macromolecules constructed from many linked amino acids Some amino acids are hydrophobic and so mix with the inside of the cell membrane These proteins sit inside the cell membrane

13. Membrane Protein Functions Cell membrane proteins serve many vital functions: – Markers – Receptors – Channels Transporting things in and out of cells is a key function of life! – Will cover this more tomorrow

14. Parts of the Eukaryotic Cell (organelles) Nucleus Cell membrane Cytoskeleton Cytoplasm Mitochondria Ribosome Endoplasmic Reticulum Golgi Apparatus

15. Nucleus: The Library The nucleus stores all the cell's genetic information in massive molecules of DNA The DNA molecules are packed into many pairs of chromosomes When a protein is needed, signals enter the nucleus and cause copies of the appropriate genes to be made with RNA

16. Key Nucleus Facts: Every complete human cell has one nucleus Exception: red blood cells have no nucleus (also have no other membrane-bound organelles) Exception: muscle cells are many cell fused together and so have many nuclei Every nucleus has the complete human genome The nucleus has its own envelope to keep unwanted things out

17. Cytoskeleton: The City Streets Every cell has long strands of different kinds of proteins running through it When organelles are moved they move along the cytoskeleton If the cell moves under its own power it uses the cytoskeleton as well

18. Key Cytoskeleton Facts: There are several different types of fibers and they have different functions All cells must produce each of these fibers These fibers are made of different kinds of proteins (keratin, collagen, actin, myosin, etc) If the genes for any of these fibers are no good, no cells can form and the organism will never develop, grow or be born This makes these genes essential for life

19. Cytoplasm: The Marketplace The liquid of the cell is called cytosol Cytosol plus the organelles are called cytoplasm All the cell's chemical reactions and growth take place here Many exchanges are made Many chemicals are stored in vesicles in the cytoplasm

20. Key Cytoplasm Facts: Cytosol is a clear liquid but it is filled with salts and sugars Cytoplasm is held within the cell membrane If the cell membrane develops a hole, cytoplasm can leak out and the cell can die If the cell membrane bursts all at once, this is called lysis and is instant cell death

21. Ribosome: The Machine Shop Site of protein synthesis Some are free floating in cytoplasm Some are attached to membranes called the endoplasmic reticulum Made with a combination of protein and RNA Probably the first structure in the original cells

22. Key Ribosome Facts Ribosome is the site for proteins to be synthesized That process will be discussed next week Cytoplasmic ribosomes make the cytoskeleton and cell enzymes Rough endoplasmic reticulum produces proteins that will enter the membrane or leave the cell Smooth endoplasmic reticulum is for synthesis of other macromolecules such as lipids

23. Golgi Apparatus: The Shipping Center Vesicles from the endoplasmic reticulum are organized here Different vesicles have different destinations – The chemical reactions that determine where these vesicles go occur here

24. Key Golgi Facts The Golgi manipulates cell membrane material into many types of vesicles – Peroxisomes contain peroxides – Lysosomes contain destructive enzymes (that can lyse chemicals or even other cells) – Secretory vesicles are designed for exocytosis (they will secrete their contents)

25. Mitochondria: The Power Plant Mitochondria (singular: mitochondrion) produce the cell's supply of energy molecules – Cells use a molecule called ATP – Made using the energy from blood sugar Must have oxygen to work – Process that requires oxygen is called aerobic

26. Key Mitochondria Facts: Mitochondria were once bacteria Captured/adopted by animal cells billions of years ago and now we work together Mitochondria must have oxygen to burn sugar Produce CO 2 as a waste product This is why we breathe in oxygen and breathe out carbon dioxide Mitochondria sustain our high metabolic rate Cyanide blocks mitochondrial activity, which is why it is a lethal poison to us

27. Mitochondria Activity Mitochondria perform aerobic respiration (requiring O 2 ) – This is the key part of cellular respiration that will be covered next week Prokaryotes have no membrane-bound organelles, therefore they do not have mitochondria – Some bacteria are aerobic, which means they perform the same processes as a mitochondrion!

28. See you in lab!