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Genetic Reduction of Inositol Triphosphate (InsP 3 ) Increases Tolerance of Tomato Plants to Oxidative Stress Mohammad Alimohammadi 1, Mohamed H. Lahiani.

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Presentation on theme: "Genetic Reduction of Inositol Triphosphate (InsP 3 ) Increases Tolerance of Tomato Plants to Oxidative Stress Mohammad Alimohammadi 1, Mohamed H. Lahiani."— Presentation transcript:

1 Genetic Reduction of Inositol Triphosphate (InsP 3 ) Increases Tolerance of Tomato Plants to Oxidative Stress Mohammad Alimohammadi 1, Mohamed H. Lahiani 1 and Mariya V. Khodakovskaya 1 * 1 Department of Biology, University of Arkansas at Little Rock *Author for correspondence: mvkhodakovsk@ualr.edumvkhodakovsk@ualr.edu Tel: 501- 683-2656 Fax: 501-569-3271

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3 Figure S1: Transcript profile of genes expressed differently in InsP 5-ptase transgenic line (L7) and with wild- type (WT) exposed to continuous light (50 µmol m -2 s -1 light intensity at 35 °C) for 7 days. Genes are clustered by hierarchical clustering (Euclidean distance). Two independent biological replicates for each plant line are presented here.

4 a b Figure S2: Relative transcript abundance of (a) PR protein (Les.3408.1.S1_at) and (b) wound- induced proteinase inhibitor II prepeptide (Les.1675.1.S1_at) in tomato wild-type (WT) and InsP 5-tase transgenic line L7 exposed to continuous light (50 µmol m-2 s-1 light intensity at 35 °C) for 7 days. Gene expression was analyzed by qRT-PCR. Results are demonstrated as the average of two independent biological replicates. Relative expression levels were normalized to an internal standard (18S transcript) for each treatment. Bars represent standard error (SE) of means.

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35S P.tAPX or GUS intronNOS T. G P.XVET3A Lex A P.ccdB G P.XVET3A Lex A P. tAPX or GUS intron Xba I pGWB80/tAPX (or GUS) pMDC7 A B C pMDC7/tAPX.
Figure S1. Genomic PCR of in vitro potato plants transformed with StPTB1 prom (top) and StPTB6 prom (bottom) constructs using nptII-specific primers. Thirty.

Figure S1. Genomic PCR of in vitro potato plants transformed with StPTB1 prom (top) and StPTB6 prom (bottom) constructs using nptII-specific primers. Thirty.
Supplemental figure 1: Correlation coefficients between signal intensities from biological replicates of wild.

Supplemental figure 1: Correlation coefficients between signal intensities from biological replicates of wild.
Supplementary Fig. S1: Hierarchical clustering of AUX/IAA genes, showing significant differential expression under at least one abiotic stress condition.

Supplementary Fig. S1: Hierarchical clustering of AUX/IAA genes, showing significant differential expression under at least one abiotic stress condition.
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Figure SOM1. Functional roles of the genes affected in zmet2-m1 mutants. Although the genes localized on the intracellular membranes were slightly over-represented.
Gene expression (signal intensity) Control Osmotic Salt Drought Root 0 200 400 600 800 Control 0.5 13 612 24 Gene expression (signal intensity) Treatment.

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Supplementary Fig. S1: GO classification of 2,318 differentially expressed genes during fruit ripening in tomato. The genes which show differential expression.
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Supplementary data 2: Reproducibility of metabolite MSI Reproducibility of MSI data was evaluated by mean ± standard deviation (5 biological replications).
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Supplementary Material

Supplementary Material
S8 Figure. Expression of genes determined by qPCR matches expression determined from transcriptome assembly. Genes encoding adenylate cyclase, triacylglycerol.

S8 Figure. Expression of genes determined by qPCR matches expression determined from transcriptome assembly. Genes encoding adenylate cyclase, triacylglycerol.
Additional file 8: Estimation of biological variations

Additional file 8: Estimation of biological variations
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Microtubule perturbations affect pathway variability η2(P) and transmitted signal P at or upstream of the Ste5 recruitment step Microtubule perturbations.
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Fig. S12. Hierarchical tree graph of overrepresented GO terms in biological process category in 18O w.r.t. WT under 100mM NaCl at 24 h stress comparative.
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