1. Cell Biology 1389-90 Cell Biology 1389-90 S. Rahgozar,PhD University of Isfahan Faculty of Science 3. Vital and non-vital staining 1

2. Vital Staining  Advantage To assess functional activity/ cell physiology a dye or stain capable of penetrating living cells or tissues and not inducing immediate evident degenerative changes—called also vital dye  Definition

4.  Classification o Basic o Acidic o Amphoteric o Electroneutral Common Vital stains  Eosin to stain cytoplasm, collagen, muscle fibers

5.  Propodium iodide DNA stain that can differentiate necrotic, apoptotic and normal cells  Methylene blue a living-cell exclusion dye. A differential stain.  Erythrosine Red No. 3, used for food colouring  Methyl green to stain the chromatin of the cell  Methyl blue to stain collagen, cell wall in fungi.

6.  Neutral red to stain Lysosomes Can be used for staining Golgi apparatus in cells and Nissl granules in neurons.  Janus green to stain mitochondria changes colour according to the amount of oxygen present. Oxygen present: Indicator oxidised to green-blue colour Oxygen absent: Indicator reduced to pink colour