1. Prokaryotes

2. Prokaryotes – aka Bacteria –Prokaryotes are found wherever there is life, have a collective biomass that is at least ten times that of all eukaryotes, thrive in habitats too cold, too hot, too salty, too acidic, or too alkaline for any eukaryote, cause about half of all human diseases, and are more commonly benign or beneficial.

3. The Structure and Function of Prokaryotes –Prokaryotic cells lack a membrane-enclosed nucleus, lack other membrane-enclosed organelles, typically have cell walls exterior to their plasma membranes, but display an enormous range of diversity.

4. The Structure of Prokaryotes Plasma membrane Cell wall Capsule Prokaryotic flagellum Ribosomes Nucleoid Pili

5. Identifying Prokaryotes * Prokaryotes are identified by: –Their shapes –The chemical natures of their cell walls –The ways they move

6. Cell Shape Three shapes possible: Rod shaped – called bacilli Sphere-shaped – called cocci Spiral-shaped – called spirilla Can form chains or clumps/clusters – use these prefixes: Diplo = pair Strepto = chain Staphlyo = cluster/bunch –Ex. Diplobacilli = pair of rod-shaped bacteria –Ex. Streptococci = chain of sphere-shaped bacteria –Ex. Staphlyococci = cluster/bunch of sphere-shaped bacteria

7. Cell Wall Gram staining – 2 stains: 1 violet, 1 red –Bacteria stain purple – Gram positive – cell wall contains mainly peptidoglycan –Bacteria stain red – Gram negative – have second, outer, layer of lipid and carbohydrate molecules

8. Bacterial Movement –Flagella – whip-like structures for movement –Slime-like material like a slug –May not move at all

9. Growth and Reproduction Can divide every 20 minutes! Growth held in check by food and waste products (think limiting factors) Binary fission – just like cell division – two “daughter” cells – asexual reproduction Conjugation – transfer genetic material from one cell to another – increases genetic diversity Spore formation – form endospores when conditions are unfavorable for growth

10. Obtaining Energy Autotrophs –Photoautotrophs – found where light is plentiful (surfaces of lakes, streams, oceans) –Chemoautotrophs – energy from inorganic molecules – chemical reactions (hydrothermal vents on ocean floor) Heterotrophs –Most bacteria are heterotrophs, like humans – may even compete with humans for food – can lead to food poisoning –Photoheterotrophs – combine autotrophic and heterotrophic styles

11. Releasing Energy Respiration – requires oxygen (O 2 ) – obligate aerobes Fermentation – absence of oxygen (O 2 ) – obligate anaerobes Facultative Anaerobes – can live with or without oxygen (O 2 ) – can switch between respiration and fermentation = grow just about anywhere

12. The Two Main Branches of Prokaryotic Evolution: Bacteria and Archaea 2 Kingdoms – used to be just be one - Monerans - By comparing diverse prokaryotes at the molecular level, biologists have identified two major branches of prokaryotic evolution: –Bacteria – larger of two kingdoms; live almost everywhere, E. coli, cell walls contain peptidoglycan –Archaebacteria – cell walls lack peptidoglycan, may be ancestors of eukaryotes, live in extremely harsh environments (oxygen-free, salty, hot)

13. Bacteria and Disease –Bacteria and other organisms that cause disease are called pathogens. –Most pathogenic bacteria produce poisons. ****Disease – some bacteria damage the tissues of the infected organism directly by breaking them down for food. Others release toxins (poisons) that harm the body.**** Vaccines can prevent bacterial infections (ex. tetanus shots). Antibiotics block growth and reproduction of bacteria; cure many bacterial diseases; sanitation important

14. Common Diseases Caused by Bacteria Tooth decay Lyme disease Tetanus Tuberculosis Salmonella food poisoning Pneumonia Cholera Streptococcus mutans Borrelia burgdorferi Clostridium tetani Mycobacterium tuberculosis Salmonella enteritidis Streptococcus pneumoniae Vibrio cholerae Regular dental hygiene Protection from tick bites Current tetanus vaccination Vaccination Proper food-handling practices Maintaining good health Clean water supplies DiseasePathogenPrevention

15. Bacteria in Nature –Pathogenic bacteria are in the minority among prokaryotes. –Far more common are species that are essential to our well-being, either directly or indirectly. –* Bacteria are vital to maintaining the living world. Some are producers that capture energy by photosynthesis. Others help to break down the nutrients in dead matter and the atmosphere, allowing other organisms to use the nutrients.

16. Bacteria in the Environment - Benefits Decomposers – nutrient/chemical recycling (dead trees, leaves, etc.) Nitrogen fixation – convert pure nitrogen (N) to compounds that can be used by other organisms –Ex. Clover plants and nodules on roots with bacteria inside Symbiotic relationships with other organisms –Us – E. coli and our intestines –Cattle – bacteria to help them digest cellulose

17. Human Uses of Bacteria Food – cheese, yogurt, buttermilk, sour cream, pickles, vinegar, some bread Industry – Bioremediation - digest oil spills, remove waste products/poisons in the water, sewer treatments, synthesize drugs and chemicals

18. Controlling Bacteria Refrigeration, canning, sterilization, chemical treatments Disinfectants used on surfaces outside the body