1. TUES 4/30 wk-6 OBJECTIVE: 11 TOPIC: genetic technology DO NOW :  daily grade: -12.4 READ-APP/Qs AGENDA: WRAP UP :  DW: -13.2 pre-LAB: VIRTUAL ELECTROPHORESIS DUE: BLOCK  12.3 mini-EXAM—this FRI!  -WK#5 OBJECTIVE QUIZ—MON (abs: before/after school) -12.3 REVIEW - 13.2 READ Qs -13.2 PRE-LAB: ELECTROPHORESIS  MON: -GATTACA Qs -wk #5 org/OBJ QUIZ  ABSENT--  HANDOUTS: -13.2 virtual electrophoresis -protein concept map  COMPLETE & TURN IN: -GATTACA Qs -wk #5 ORG/OBJ QUIZ BEFORE/AFTER SCHOOL: -DW check—12 materials - -protein concept map A:-see next slide general/honors

2. DO NOW

3. TRANSCRIPTION TRANSLATION mRNA CYTOPLASM NUCLEUS CYTOPLASM PROTEINS AMINO ACIDS

4. 2007-2008 FLOW CHART  TRANSCRIPTION/TRANSLATION http://www.youtube.com/watch?feature=player_embedded&v=NJxobgkPEAo From RNA to Protein Synthesis - YouTube NUCLEUS CYTOPLASM RIBOSOME DNA mRNA rRNA tRNA RNA polymerase Amino Acid Polypeptide chain [PROTEIN] TRANSLATION TRANSCRIPTION

5. FLOW CART TRANSCRIPTION TRANSLATION ---------------------------------------------------------- NUCLEUSCYTOPLASM RNA polymerase mRNA [code] tRNA [interpret] polypeptide chain / PROTEIN rRNA a.a

6.  TRANSCRIPTION/TRANSLATION

7.  PROTEIN PROTEIN STRUCTURE

8. 12.4 GENE REGULATIONS and MUTATIONS  WHAT YOU WILL LEARN -prokaryotic/eukaryotic gene regulation -types of mutations & results -mutagens

9. READING Q’s 1-Like DNA code, the following sentence: “The dog ran” contains only 3 letter words -INSERT a “z” after “Th”—rewrite the sentence: Thz edo gra -WRITE the sentence again inserting an additional letter Thz zed ogr -What happens to the sentences as you add letters? become nonsense

10. READING Q’s 2-DESCRIBE point mutation -single nucleotide/base pair is changed can cause a genetic disorder

11. READING Q’s 3-NAME -what type of mutation occurs when a STOP codon replaces an amino acid codon -nonsense mutation cause translation to terminate early proteins cannot function normally

12. READING Q’s 4-EVALUATE -Would a 3-nucleotide insertion result in a frameshift?  YES  NO -EXPLAIN -insertion of a nucleotide shifts an entire 3 code sequence—shifts amino acid sequence X

13. READING Q’s 5-RECORD The mRNA sequence/sub A for G -AUG–AAG–UUU–GGC – ACA – UUG-UAA

14. READING Q’s 6-COMPLETE table: frameshift / substitution -substitution -frameshift

15. READING Q’s 7-EXPLAIN how UV light damages DNA -links thymines—forms a “kink”

16. READING Q’s 8-DETERMINE -what a mutation can do to a body (somatic) cell -cell death -cancer cell

17. READING Q’s 9-DISCUSS why a mutagen can have longer lasting effects in a sex cell than a body cell -mutations in sex cells are passed on to their offspring -mutations in body cells are not inherited