1. Butanol Producing E. Coli and Algae biofuels Austin Pruitt

3. The Plan

4. Chlorella Vulgaris

5. Bump Array Sorts Particles by size Aids in evolutionary acceleration

6. E. Coli

7. Death Galaxy The Death Galaxy is a microchip device with interconnected wells and gradient Mimics heterogeneous microenvironments Can result in rapid evolution

8. Evolutionary Dynamic Principles  Stress gradient and microenvironments should yield rapid evolution  Low stress allows reproduction  Mutated cells climb the gradient  Small population size in wells allows faster fixation

9. E. Coli Resistance to Cipro

10. Progress so far

11. Next Steps  Begin UV and Bump array testing  Run several death galaxies simultaneously  Attempt to evolve bacteria/algae to thrive in different conditions

12. Conclusion  Gained many skills through this experience  Ongoing research  Better understanding of evolutionary dynamics, and fluid mechanics

13. Thank you!

14. Sources  http://www.princeton.edu/physics/puchalla/research/microfluidics /BumpArray_1.jpg  http://harmonicarts.ca/wp- content/uploads/2013/04/chlorella_vulgaris_beij_1_0.jpg  http://krisdedecker.typepad.com/photos/uncategorized/2008/04/ 04/algae1.jpg  http://ak2.picdn.net/shutterstock/videos/4077487/preview/stock- footage-moscow-june-cars-stands-in-traffic-jam-on-the-city-center- june-moscow-russia.jpg  http://innovativesolarsystemsllc.com/wp-content/uploads/solar- power-generator.jpg  http://s.hswstatic.com/gif/hydropower-plant-usbr-hoover-1.jpg  http://cleantechnica.com/files/2014/04/wind-turbines17.jpg http://cleantechnica.com/files/2014/04/wind-turbines17.jpg  http://www.prism.princeton.edu/PRISM_cleanroom/images/prismlo gosmall.jpg