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How DNA is stored in cells (what we call the tightly wound packages of DNA)
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What are chromosomes?
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The number of chromosomes in most cells in your body
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What is 46?
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A section of a chromosome that codes for the production of a particular protein
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What is gene?
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If we imagine a nucleus to be like a library filled with 46 books, what are the “chapters” of the books?
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What are genes?
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Rank the following from simplest/smallest (1) to most complex/largest (6) cell chromosome gene nitrogenous base nucleotide nucleus
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What is: nitrogenous base (1) nucleotide (2) gene (3) chromosome (4) nucleus (5) cell (6)
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The symbols of the 4 different bases in DNA
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What are A, T, G and C
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The sugar in DNA
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What is deoxyribose sugar?
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DAILY DOUBLE!!! The components of DNA’s backbone
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What are deoxyribose sugar and phosphate molecules?
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The charge on DNA and the component that gives it that charge
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What is negative and phosphate molecules
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The type and number of bonds that join a cytosine to a guanine base
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What is 3 hydrogen bonds
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The 2 men who won the Nobel Prize for the discovery of DNA’s structure
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Who are Watson and Crick?
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The scientist whose student took this famous X-ray
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Who is Rosalind Franklin?
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What this DNA proved about DNA’s nitrogenous bases
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What is A pairs with T and C pairs with G?
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DAILY DOUBLE!!! How Chargaff’s data helped Watson and Crick
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What is it told them which bases to pair together in their model (A with T and C with G)
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If 31% of the bases in a yeast cell are A, this percent are G
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What is 19% (If 31% are A, then 31% are T, making 62% be A and T, so 38% leftover would be C & G. Half would be G and half of 38% is 19%)
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The place in the body from which cells are typically extracted for DNA analysis
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What is cheek?
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The chemical mixed with water before swishing in the mouth (in order to help break cells away from cheek
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What is salt?
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Dish soap lyses cells. The literal meaning of the work lyse is what this.
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What is break/split?
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During DNA extraction, DNA precipitates. To precipitate means this.
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What is “for a solid to form out of a solution”?
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Of this list, the item you could be missing and still extract DNA Dishsoap Ethanol Food Coloring Salt Water
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What is food coloring? (Yeah, sorry. I couldn’t think of a tough 500 pointer for this topic!)
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The name (or abbreviation for the name) of the process used to amplify a sample of DNA
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What is PCR?
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What a restriction enzyme does to a strand of DNA
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What is cuts it?
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DAILY DOUBLE!!! Where restriction enzymes come from
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What is bacteria?
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This is a thermocycler. What process is it necessary for?
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What is PCR?
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The literal meaning of the word polymerase (provided by breaking it into the 3 roots)
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What is “enzyme that makes many parts”? poly=many mer=parts ase=enzyme
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The process by which DNA fragments can be separated
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What is gel electrophoresis?
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What pulls DNA across a gel (what it is attracted to)
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What is electricity (or a positive charge)?
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During electrophoresis, RFLPs get separated based on a difference in this property
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What is SIZE?
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Based on an understanding of the process of electrophoresis, it can be inferred that phore’s literal meaning is (choose one) a)To carry b)To break c)To combine d)To repel
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What is “to carry”? (Electrophoresis literally means “the condition of being carried by electricity”)
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A segment of DNA has two restriction sites–I and II. When incubated with restriction enzymes I and II, three fragments will be formed–a, b, and c. Which of the following gels produced by electrophoresis would represent the separation and identity of these fragments?
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What is B? (B is shortest, so would go farthest and C is longest, so would travel the least)
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Write a paragraph that meaningfully connects the following terms: cells chromosomes DNA genes proteins traits
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EXAMPLE OF GOOD ANSWER: Human cells contain DNA that is tightly wound into 46 chromosomes. Each chromosome has many sections that are called genes. Each gene codes for a protein and the proteins that they code for determine an individual’s traits (height, eye color, etc).
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