1. USAFSAM IMMUNODIAGNOSTICS LABORATORY 25 March 2010 Presenters: SSgt Shoup, SSgt Eccleston Distribution Statement A: Approved for Public Release; distribution is unlimited. 311ABG/PA No. 10-083, 12MAR2010

2. Team Aerospace Begins Here! Departments of Epidemiology Laboratory First to move – Immunodiagnostics Sections of Immunodiagnostics Phasing of personnel and instrumentation Modular facility for testing Preparations both at Brooks City-Base and at Wright-Patterson AFB Transition of testing The effects of moving in relation to customer care – minimal ADVON team “We’re Moving – Not Closing”

3. Team Aerospace Begins Here! Brooks City-Base Lab Departments of the Epi Laboratory Immunodiagnostics Microbiology Virology Bacteriology Parasitology Molecular Shipping/Receiving Customer Service 3

4. Team Aerospace Begins Here! Immunodiagnostics Largest department in the Epi Laboratory Performs over 90% of patient workload Chosen to relocate first due to size of instrumentation and workload volume 4

5. PHE FY 2009 WORKLOAD Total Tests: 2,039,587 Immunodiagnostics Tests: 1,896,006 Other PHE Tests: 143,581

6. Team Aerospace Begins Here! Sections of Immunodiagnostics HIV HEPATITIS EIA BIOPLEX IFA GC/CHLAMYDIA 6

7. Team Aerospace Begins Here! Sections of Immunodiagnostics HIV Automated testing performed on Siemens Centaur XP analyzer: HIV Screens Semi-Automated testing performed using BioRad AutoBlot 3000: HIV Western Blots 7

8. Team Aerospace Begins Here! Sections of Immunodiagnostics Hepatitis Automated testing performed on Siemens Centaur Classics: Hepatitis (A, B, C) Panels Reflex testing for Hepatitis B positive results Manual HBeAg/HBeAb ELISA testing 8

9. Team Aerospace Begins Here! Siemens Centaur Classic/XP

10. Team Aerospace Begins Here! BioRad AutoBlot 3000 Semi-Automated Western Blot analyzer

11. Team Aerospace Begins Here! Sections of Immunodiagnostics Enzyme Immunoassay (EIA) Tests performed on Trinity-Biotech DSX analyzer: CMV G/M, H. Pylori, Lyme AB, Rubeola, Mumps, Varicella Tests performed on BioRad Bioplex analyzer: ANA, EBV G/M, HSV I/II, Syphilis 11

12. Team Aerospace Begins Here! Trinity DSX and BioRad Bioplex

13. Team Aerospace Begins Here! Sections of Immunodiagnostics Immunofluorescent Assay (IFA) Confirmatory methods performed: CMV M and WB Lyme M Manual tests performed: Auto- Antibody, Coccidioides, FTA-ABS, Mycoplasma, Q Fever G/M, Rickettsia, VDRL 13

14. Team Aerospace Begins Here! Sections of Immunodiagnostics Miscellaneous Testing Automated testing performed on Gen-Probe Tigris analyzer: Gonorrhea/Chlamydia Automated testing performed on Siemens Centaur XP analyzer: Rubella G/M, Toxoplasma, Thyroid AB 14

15. Team Aerospace Begins Here! Gen-Probe Tigris

16. Team Aerospace Begins Here! BRAC Personnel Movement of military and civilian personnel in 3 phases Phase 1– ADVON team 6 mil, 2 civ PCS June 2010, Lab Director PCS Oct 2010 Phase 2– 3 mil, 2 civ PCS December 2010 Phase 3– 2 civ PCS January 2011 TDY– 2 civ for validation studies on instrumentation 16

17. Team Aerospace Begins Here! BRAC Analyzers Movement of instrumentation in 2 phases Phase 1 – July 2010  2 Bioplex analyzers (1 from BCB to WP,1 new one from BioRad)  2 Tigris analyzers – brand new from GenProbe  4 Centaur analyzers – brand new from Siemens 17

18. Team Aerospace Begins Here! BRAC Analyzers Movement of instrumentation in 2 phases Phase 2 – Jan 2011  1 Bioplex analyzer (BCB)  1 Tigris analyzer (BCB) 18

19. Team Aerospace Begins Here! Preparations while at BCB Upfront plans completed prior to move Interop CHCS test files Contracts – Equipment (new and reagent rental, Personnel, Supplies) Accreditations (CAP, CDC, etc.) OIs/SOPs, Policies, Procedure s

20. Team Aerospace Begins Here! Preparation for Testing at WP 20 Moving trucks arrive Jul 2010 Unpacking/Laboratory Set-up Computer set-up/access Vendors and analyzers Validation Vendors Technical Supervisors Initial testing scheduled to begin Oct 2010

21. Team Aerospace Begins Here! Testing Samples Transition of patient samples to WP Timeline 30% – 15 Oct 2010 60% - 15 Nov 2010 100% - 15 Dec 2010 Volunteers First come, first serve! CAP Inspection 21

22. Team Aerospace Begins Here! Streamlined Process 22 Dual-Operations Full transition to Wright-Patterson 6 month process Turn-Around Times Maintained Modular Bldg 18-month contract – Jan 2012 Transition to new facility after everyone else Instrumentation

23. Team Aerospace Begins Here! Streamlined Process 23 Modular USAFSAM

24. Team Aerospace Begins Here! Summary 24 Breakdown of the Epi labs departments and sections Analyzers used for testing Transition of personnel and equipment to Wright-Patterson Testing samples and streamlining the ability to perform dual-operations Your ADVON team members

25. Team Aerospace Begins Here! ADVON Team Members 25 Back: TSgt Mario Sulit, SSgt Andrew Eccleston, SSgt LeVaughn Grant, Clay Huddleston Front: Lt Col Kathy Taylor, SSgt Jennifer Shoup, Annette Compton, SSgt Robert Bennett, Elsie Martinez

26. Team Aerospace Begins Here! Questions? 26 United States School of Aerospace Medicine Epidemiology Laboratory Service (USAFSAM/PHE) 2730 Louis Bauer Drive Brooks City-Base, TX 78235-5132 EPI Lab Customer Service Team DSN: 240-8378 Commercial: 210.536.8378 Email: epilab.help@brooks.af.mil Website: https://kx.afms.mil/epi/