1. Using the feature color file (.fc) and fasta file to create figures (ATOH7 example) Using the feature color file (.fc) and fasta file to create figures (ATOH7 example)

2. Save both files on your computer e.g. right click (1) and Save as... (2) Save both files on your computer e.g. right click (1) and Save as... (2) 1 2 ATOH7_NM_145178PromoterBlock_3.fcATOH7_NM_145178PromoterBlock_3.fasta

3. Open Jalview en click File » Input Alignment » from file (Jalview is available at www.jalview.org) Open Jalview en click File » Input Alignment » from file (Jalview is available at www.jalview.org)

4. Open the.fasta file

6. Open the feature color (.fc) file

9. Open the feature settings to turn binding sites on and off...

11. Click File » Export Image and choose the output format or “Print” to PDF if you have e.g. the free PDFCreator installed Click File » Export Image and choose the output format or “Print” to PDF if you have e.g. the free PDFCreator installed