1. Gene analysis Nucleotide BLAST : http://blast.ncbi.nlm.nih.gov/Blast.cgihttp://blast.ncbi.nlm.nih.gov/Blast.cgi - 유전자 염기서열을 가지고 데이터베이스에 등록되어 있는 서열과 비교하여 유사한 서열을 찾아줌 Protein BLAST : http://blast.ncbi.nlm.nih.gov/Blast.cgihttp://blast.ncbi.nlm.nih.gov/Blast.cgi - 아미노산서열을 가지고 데이터베이스에 등록되어 있는 서열과 비교하여 domain 및 유사 서열 찾아줌 Blastx : http://blast.ncbi.nlm.nih.gov/Blast.cgihttp://blast.ncbi.nlm.nih.gov/Blast.cgi - 유전자 염기서열을 아미노산 서열로 바꿔 유사 아미노산 서열을 찾아줌 Dnassist version 2.2 program - 유전자 혹은 아미노산 서열 비교, ORF 찾을때, 제한효소 site 확인시, 물리적 특성 등 ClustalW multiple alignment 1.8 program - 유전자 혹은 아미노산 서열 비교 MEGA 3.1 - 계통도 작성시 사용 SignalP program : http://www.cbs.dtu.dk/services/SignalP/http://www.cbs.dtu.dk/services/SignalP/ - 신호서열을 찾아줌 LipoP 1.0 server : http://www.cbs.dtu.dk/services/LipoP/http://www.cbs.dtu.dk/services/LipoP/ - lipoprotein site 찾아줌 ( 그람 음성균만 해당 ) Motif scan prediction program : http://myhits.isb-sib.ch/cgi-bin/motif_scanhttp://myhits.isb-sib.ch/cgi-bin/motif_scan - 모티브들을 찾아줌 TMHMM server v.2.0 : http://www.cbs.dtu.dk/services/TMHMM/http://www.cbs.dtu.dk/services/TMHMM/ - transmembrane ( 막에 붙는 단백질 ) site 찾아줌 NetNGlyc 1.0 server : http://www.cbs.dtu.dk/services/NetNGlyc/http://www.cbs.dtu.dk/services/NetNGlyc/ - N glycosilation site 찾을 때 사용 ( 진핵세포만 해당 ) NetOGlyc 3.1 server : http://www.cbs.dtu.dk/services/NetOGlyc/http://www.cbs.dtu.dk/services/NetOGlyc/ - O glycosilation site 찾을 때 사용 ( 진핵세포만 해당 ) Oligo Analyzer 3.1 : http://eu.idtdna.com/analyzer/Applications/OligoAnalyzer/http://eu.idtdna.com/analyzer/Applications/OligoAnalyzer/ - primer 제작시 Tm, GC% 등 설정

2. ATGAAGATTAAATTTTTATCTGCAGCAATCGCTGCAAGCTTAGCATTGCCATTAAGTGCTGCTACCTTAGTCACCTCTTTTGAGGAAGCCGACTA CAGCAGCTCTGAAAACAATACTGAATTCTTGGAAGTGTCTGGAGATGCCACTTCTGAAGTTTCAACTGAACAAGCTACCGATGGTAATCAATCCA TTAAAGCGTCTTTTGACGCGGCTTTCAAACCAATGGTTGTTTGGAACTGGGGAAGTTGGAACTGGGGCGCTGAAGATGTGATGTCAGTAGATGT TGTTAACCCTAACGACACTGACGTCACCTTTGCTATTAAGCTAATTGATAGTGATATTCTTCCTGATTGGGTAGATGAGTCTCAAACCTCATTGGA CTACTTTACGGTTTCAGCTAATACCACGCAGACCTTTAGCTTTAACTTAAATGGCGGCAACGAGTTCCAAACTCATGGCGAAAACTTTAGTAAAG ATAAAGTTATCGGTGTGCAGTTCATGCTCTCTGAAAACGATCCTCAAGTGTTGTACTTTGACAACATTATGGTTGATGGCGAAACAGTCACTCCG CCACCAAGTGATGGTGCAGTGAATACACAAACCGCGCCTGTAGCCACCTTAGCGCAAATCGAAGACTTTGAAACCATTCCAGATTACTTACGAC CTGATGGTGGGGTAAACGTTTCAACTACTACTGAGATTGTGACTAAAGGCGCTGCAGCAATGGCTGCCGAGTTTACTGCAGGTTGGAACGGTTT AGTGTTTGCAGGTACTTGGAATTGGGCTGAACTAGGTGAACACACCGCAGTTGCCGTTGACGTTTCAAATACTAGCGATAGCAATATCTGGTTG TACTCACGTATCGAAGATGTAAATAGCCAAGGCGAAACAGCGACTCGCGGCGTATTGGTTAAAGCTGGCGAATCGAAAACCATCTACACCAGCT TAAATGACAACCCTTCATTGCTTACTCAAGATGAGCGCGTGTCAGCTTTAGGTTTACGTGATATTCCAGCTGACCCAATGAGCGCTCAAAATGGC TGGGGTGATTTTGTTGCTTTAGACAAATCTCAAATTACCGCTATTCGTTACTTCATTGGCGAATTAGCCAGCGGTGAGACTAGCCAAACACTTGT GTTTGATAACATGCGTGTGATTAAAGACCTTAACCACGAATCAGCCTATGCAGAAATGACTGATGCTATGGGGCAAAACAACTTAGTCACTTATG CAGGTAAAGTTGCCAGCAAAGAAGAGTTAGCTAAGTTAAGTGATCCGGAAATGGCTGTTTTGGGTGAGTTAACCAATCGCAATATGTACGGTGG TAACCCAGATTCGTCGCCAACTACAGACTGTGTGCTCGCTACGCCTGCCTCGTTTAACGCTTGTAAAGACGCTGATGGTAACTGGCAATTGGTA GACCCTGCTGGTAATGCGTTCTTCTCAACCGGTGTTGATAACATTCGTTTGCAAGATACTTACACCATGACCGGCGTGTCGAGTGACGCCGAAT CTGAGTCTGCACTTCGCCAGTCAATGTTTACAGAAATTCCAAGTGATTATGTAAATGAAAACTATGGCCCTGTGCATAGTGGACCTGTTTCTCAA GGCCAAGCTGTAAGTTTTTACGCTAATAACTTAATTACCCGCCACGCTAGCGAAGACGTATGGCGAGACATTACTGTTAAGCGCATGAAAGACT GGGGCTTTAACACCTTAGGTAACTGGACCGATCCAGCGTTGTATGCAAACGGTGACGTTCCTTACGTGGCAAATGGTTGGTCAACCTCTGGTGC CGATCGTCTTCCCGTTAAACAAATTGGCAGCGGCTACTGGGGACCACTTCCTGATCCGTGGGATGCTAACTTTGCTACCAATGCCGCCACAATG GCTGCAGAGATCAAAGCTCAGGTTGAAGGCAACGAAGAGTACTTAGTGGGTATTTTTGTTGATAACGAAATGAGCTGGGGCAATGTCACTGATG TTGAAGGCTCTCGTTATGCGCAAACGCTAGCAGTGTTCAATACCGACGGCACTGATGCAACAACTAGCCCTGCTAAAAATAGCTTTATTTGGTTC TTAGAAAACCAGCGTTATACCGGTGGCATTGCTGACCTAAACGCAGCCTGGGGAACCGATTATGCGTCTTGGGATGCGATGCGCCCAGCGCAA GAGTTAGCTTATGTGGCTGGCATGGAAGCTGATATGCAGTTCCTTGCTTGGCAGTTTGCGTTCCAATACTTCAACACCGTAAACACGGCATTAAA AGCTGAGTTACCAAACCACTTGTACTTGGGCTCTCGCTTTGCAGATTGGGGACGTACTCCTGATGTAGTAAGTGCTGCTGCGGCTGTTGTTGAT GTGATGAGTTACAACATCTACAAAGACAGTATTGCAGCTGCCGATTGGGATGCTGATGCCTTAAATCAAATTGAAGCCATTGATAAGCCAGTAAT TATTGGTGAGTTCCACTTCGGTGCGCTTGATAGCGGTTCGTTTGCAGAAGGTGTAGTAAATGCCACTTCGCAACAAGATCGTGCAGACAAAATG GTTAGCTTCTACGAATCAGTAAATGCCCATAAAAACTTTGTAGGTGCGCATTGGTTCCAATACATCGATTCACCATTAACGGGTCGTGCATGGGA TGGCGAGAACTACAACGTTGGTTTTGTTAGCAATACTGACACGCCATATACATTGATGACAGATGCTGCGCGTGAGTTTAACTGTGGTATGTACG GCACTGACTGCTCTAGCTTAAGCAATGCTACTGAAGCTGCTTCGAGAGCCGGTGAGTTGTATACCGGTACCAATATTGGTGTTAGCCACTCTGG CCCAGAAGCGCCAGATCCAGGTGAGCCAGTTGATCCTCCAATTGATCCGCCAACACCACCAACAGGTGGCGTAACTGGCGGTGGCGGTAGCG CAGGTTGGTTATCGCTACTAGGTTTGGCCGGCGTATTTTTACTAAGACGTCGTAAAGTGTAA AG17 agarase - ORF

3. ATGGTTGTTTGGAACTGGGGAAGTTGGAACTGGGGCGCTGAAGATGTGATGTCAGTAGATGTTGTTAACCCTAACGACACTGACGTCAC CTTTGCTATTAAGCTAATTGATAGTGATATTCTTCCTGATTGGGTAGATGAGTCTCAAACCTCATTGGACTACTTTACGGTTTCAGCTAATA CCACGCAGACCTTTAGCTTTAACTTAAATGGCGGCAACGAGTTCCAAACTCATGGCGAAAACTTTAGTAAAGATAAAGTTATCGGTGTGC AGTTCATGCTCTCTGAAAACGATCCTCAAGTGTTGTACTTTGACAACATTATGGTTGATGGCGAAACAGTCACTCCGCCACCAAGTGATG GTGCAGTGAATACACAAACCGCGCCTGTAGCCACCTTAGCGCAAATCGAAGACTTTGAAACCATTCCAGATTACTTACGACCTGATGGT GGGGTAAACGTTTCAACTACTACTGAGATTGTGACTAAAGGCGCTGCAGCAATGGCTGCCGAGTTTACTGCAGGTTGGAACGGTTTAGT GTTTGCAGGTACTTGGAATTGGGCTGAACTAGGTGAACACACCGCAGTTGCCGTTGACGTTTCAAATACTAGCGATAGCAATATCTGGTT GTACTCACGTATCGAAGATGTAAATAGCCAAGGCGAAACAGCGACTCGCGGCGTATTGGTTAAAGCTGGCGAATCGAAAACCATCTACA CCAGCTTAAATGACAACCCTTCATTGCTTACTCAAGATGAGCGCGTGTCAGCTTTAGGTTTACGTGATATTCCAGCTGACCCAATGAGCG CTCAAAATGGCTGGGGTGATTTTGTTGCTTTAGACAAATCTCAAATTACCGCTATTCGTTACTTCATTGGCGAATTAGCCAGCGGTGAGA CTAGCCAAACACTTGTGTTTGATAACATGCGTGTGATTAAAGACCTTAACCACGAATCAGCCTATGCAGAAATGACTGATGCTATGGGGC AAAACAACTTAGTCACTTATGCAGGTAAAGTTGCCAGCAAAGAAGAGTTAGCTAAGTTAAGTGATCCGGAAATGGCTGTTTTGGGTGAGT TAACCAATCGCAATATGTACGGTGGTAACCCAGATTCGTCGCCAACTACAGACTGTGTGCTCGCTACGCCTGCCTCGTTTAACGCTTGT AAAGACGCTGATGGTAACTGGCAATTGGTAGACCCTGCTGGTAATGCGTTCTTCTCAACCGGTGTTGATAACATTCGTTTGCAAGATACT TACACCATGACCGGCGTGTCGAGTGACGCCGAATCTGAGTCTGCACTTCGCCAGTCAATGTTTACAGAAATTCCAAGTGATTATGTAAAT GAAAACTATGGCCCTGTGCATAGTGGACCTGTTTCTCAAGGCCAAGCTGTAAGTTTTTACGCTAATAACTTAATTACCCGCCACGCTAGC GAAGACGTATGGCGAGACATTACTGTTAAGCGCATGAAAGACTGGGGCTTTAACACCTTAGGTAACTGGACCGATCCAGCGTTGTATGC AAACGGTGACGTTCCTTACGTGGCAAATGGTTGGTCAACCTCTGGTGCCGATCGTCTTCCCGTTAAACAAATTGGCAGCGGCTACTGGG GACCACTTCCTGATCCGTGGGATGCTAACTTTGCTACCAATGCCGCCACAATGGCTGCAGAGATCAAAGCTCAGGTTGAAGGCAACGAA GAGTACTTAGTGGGTATTTTTGTTGATAACGAAATGAGCTGGGGCAATGTCACTGATGTTGAAGGCTCTCGTTATGCGCAAACGCTAGCA GTGTTCAATACCGACGGCACTGATGCAACAACTAGCCCTGCTAAAAATAGCTTTATTTGGTTCTTAGAAAACCAGCGTTATACCGGTGGC ATTGCTGACCTAAACGCAGCCTGGGGAACCGATTATGCGTCTTGGGATGCGATGCGCCCAGCGCAAGAGTTAGCTTATGTGGCTGGCA TGGAAGCTGATATGCAGTTCCTTGCTTGGCAGTTTGCGTTCCAATACTTCAACACCGTAAACACGGCATTAAAAGCTGAGTTACCAAACC ACTTGTACTTGGGCTCTCGCTTTGCAGATTGGGGACGTACTCCTGATGTAGTAAGTGCTGCTGCGGCTGTTGTTGATGTGATGAGTTAC AACATCTACAAAGACAGTATTGCAGCTGCCGATTGGGATGCTGATGCCTTAAATCAAATTGAAGCCATTGATAAGCCAGTAATTATTGGT GAGTTCCACTTCGGTGCGCTTGATAGCGGTTCGTTTGCAGAAGGTGTAGTAAATGCCACTTCGCAACAAGATCGTGCAGACAAAATGGT TAGCTTCTACGAATCAGTAAATGCCCATAAAAACTTTGTAGGTGCGCATTGGTTCCAATACATCGATTCACCATTAACGGGTCGTGCATG GGATGGCGAGAACTACAACGTTGGTTTTGTTAGCAATACTGACACGCCATATACATTGATGACAGATGCTGCGCGTGAGTTTAACTGTG GTATGTACGGCACTGACTGCTCTAGCTTAAGCAATGCTACTGAAGCTGCTTCGAGAGCCGGTGAGTTGTATACCGGTACCAATATTGGT GTTAGCCACTCTGGCCCAGAAGCGCCAGATCCAGGTGAGCCAGTTGATCCTCCAATTGATCCGCCAACACCACCAACAGGTGGCGTAA CTGGCGGTGGCGGTAGCGCAGGTTGGTTATCGCTACTAGGTTTGGCCGGCGTATTTTTACTAAGACGTCGTAAAGTG AG17 agarase – ORF( 찾기 )

4. MKIKFLSAAIAASLALPLSAATLVTSFEEADYSSSENNTEFLEVSGDAT SEVSTEQATDGNQSIKASFDAAFKPMVVWNWGSWNWGAEDVMSV DVVNPNDTDVTFAIKLIDSDILPDWVDESQTSLDYFTVSANTTQTFSF NLNGGNEFQTHGENFSKDKVIGVQFMLSENDPQVLYFDNIMVDGET VTPPPSDGAVNTQTAPVATLAQIEDFETIPDYLRPDGGVNVSTTTEIVT KGAAAMAAEFTAGWNGLVFAGTWNWAELGEHTAVAVDVSNTSDSNI WLYSRIEDVNSQGETATRGVLVKAGESKTIYTSLNDNPSLLTQDERV SALGLRDIPADPMSAQNGWGDFVALDKSQITAIRYFIGELASGETSQT LVFDNMRVIKDLNHESAYAEMTDAMGQNNLVTYAGKVASKEELAKLS DPEMAVLGELTNRNMYGGNPDSSPTTDCVLATPASFNACKDADGN WQLVDPAGNAFFSTGVDNIRLQDTYTMTGVSSDAESESALRQSMFT EIPSDYVNENYGPVHSGPVSQGQAVSFYANNLITRHASEDVWRDITV KRMKDWGFNTLGNWTDPALYANGDVPYVANGWSTSGADRLPVKQI GSGYWGPLPDPWDANFATNAATMAAEIKAQVEGNEEYLVGIFVDNE MSWGNVTDVEGSRYAQTLAVFNTDGTDATTSPAKNSFIWFLENQRY TGGIADLNAAWGTDYASWDAMRPAQELAYVAGMEADMQFLAWQFA FQYFNTVNTALKAELPNHLYLGSRFADWGRTPDVVSAAAAVVDVMS YNIYKDSIAAADWDADALNQIEAIDKPVIIGEFHFGALDSGSFAEGVVN ATSQQDRADKMVSFYESVNAHKNFVGAHWFQYIDSPLTGRAWDGE NYNVGFVSNTDTPYTLMTDAAREFNCGMYGTDCSSLSNATEAASRA GELYTGTNIGVSHSGPEAPDPGEPVDPPIDPPTPPTGGVTGGGGSA GWLSLLGLAGVFLLRRRKV Ag17 agarase protein

5. AG17 agarase -Using neural networks (NN) and hidden Markov models (HMM) trained on Gram-negative bacteria

6. The output format is essentially in GFF format. The default (long) output format looks like this: # ANIA_NEIGO SpII score=29.6052 margin=11.2327 cleavage=18-19 Pos+2=G # Cut-off=-3 ANIA_NEIGOLipoP1.0:BestSpII1129.6052 ANIA_NEIGOLipoP1.0:MarginSpII1111.2327 ANIA_NEIGOLipoP1.0:ClassSpI1118.3725 ANIA_NEIGOLipoP1.0:ClassCYT11-0.200913 ANIA_NEIGOLipoP1.0:SignalCleavII181929.6052# FALAA|CGGEQ Pos+2=G ANIA_NEIGOLipoP1.0:SignalCleavI242518.0333# GGEQA|AQAPA ANIA_NEIGOLipoP1.0:SignalCleavI202115.9259# LAACG|GEQAA ANIA_NEIGOLipoP1.0:SignalCleavI262712.0794# EQAAQ|APAET ANIA_NEIGOLipoP1.0:SignalCleavI252611.4077# GEQAA|QAPAE ANIA_NEIGOLipoP1.0:SignalCleavI27289.40252# QAAQA|PAETP Ag17 –lipoprotein site

7. CGTTAGAACGCGTAATACGACTCACTATAGGGAGACACAAGTTATGGTTGGGGTGATTGGTGTTATAATAATGGAAAC CGACGTTATATGCGTATGGGTGTAAACTGGATTAGTCCAAAACATTTTGAGTATTATATTGATGGTGAGTTAGTTAGAG TGATGTATTATAATGCAATTGCCACTAATTACAACGGAACTTGGCAATACACATATTTTAATTCTATGAATTGGAATGTA AATGGATATAATCTTCCTACTAACAACGGATCTGGATATACAGATGTAACTACTTATGCTACATCTAATGCATACGATTT TGAAAAATTAAAGGAAGCATCTAATGCATCTAACGGTTTTAATGTAATTGATCCGGCTTGGTTCCAAGGGGGAGATGA TAGTGATACAGATGGAAATGGAGTAACACAAGAGGCTAGAGGATTCACTAAAGAATTAGATATTATTATTAATATGGAA TCACAAACGTGGTTAACAGCTTCTACACCATCACAAAGTGATTTAGAAAACCCAGCGAAAAATCAAATGAAAGTAGATT GGGTACGTGTTTACAAACCTGTATCATCTAATCCAGGTTCAGATGTAGCGGTACAAAGCGTCTCTTTATCACCTGCTA ATTTAACTATGTCAGAAGGAGAGACGAGTAACTTAACAGGTAGAGTGCTGCCTTCGAATGCTACAATCCAAACAATTG CTTTTACTTCTAATAATACAAATGTAGTTTCTGTTAATCAATCAGGCTTACTAACTGCAAACGGAATTGGTACAGCAATA ATTACAGCTACATCTACAGACGGTGGTTATACTGCAACTTCTAATATTACTGTAGAGGCTGAAGATGTTGGAGGTCCA ATAAGCTCTTTAGAAATTGAAGCTGATGATTTCTCATCAACAGGCGGTACATTTAATGACGGTGTTGTTCCTTTTGGTG CAAATAAATCATCAATTGGTGTTAATTATATTAATGCTGGTGATTACATGGAATATGTAGTCGCAATTGCTGAAATGGG AGACTACTCTCTTACTTATCAAATATCTACTCCAAGTGATAATGCTAAAATTGCATGTTATGTTGATGGTAATTTAGTAG CAGATGATAATGTTCAAAACAATGGACAGTGGGATGCATACCAAGCATTAACTGCTTCTAATAATTTATCGCTAACAAC TGGTAATCATACAATTAAAATTGAAGCTTCAGGCAGCAATGATTGGCAATGGAATCTTGATAAAATGAATTTAGAAAAA TTAGGTTCAGGAACGAATCCTGAAGAACCAACGCCTCCTTTAGCCGAAGATTTTGTAATTCAAGCGGAAGACTATAAT GAAACAAGTGGTAGTTTTAATGACGGTTTTGTCCCTTTTGGTGTTAACGCATCTGCAAATGGAATTAATTATGTTAATG CAGAAGATTGGGCGGATTATGAAGTTTATCTTCCAGAGGCAGGTACATTTAACGTAACCTACACAATTGCAACGCCAA GCGATAATGCACAAATTGAAATTGTAGTCTCCCTATAGTGAGTCGTATTACGCGTTCTAGCGACAATATGTACAATCAC TAGGAATTCGCGGCCGCCTG AG31 agarase full sequence ORF

10. ag-31-LipoP

11. GGTATTTTCATAAGCTTGAGTTTGAATATGGATACAAATAATAGAAGGTACACACAAAAG AGATTGTTTCATCTAGGGCCTGTTTATCTTTCGATGATTAAATTCACAAAAGTCACTCGC ACTAGTTAAAGAAGCATATCTACATTAATTTGCATGGAGATTTTATATGAATATATTAAAA CTACTATCCTGTTCTACTTGCGCAATACTCTGCACAGCAACACATGCTGCAGATTGGGA CGCATATAGTATTCCGGCTTCTGCTGGATCAGGTAAAACATGGCAATTACAAACTGTTT CCGACCAATTTAACTACCAAGCCGGTACTTCAAATAAACCGGCAGCATTTACCAATCGT TGGAATGCTTCGTATATTAATGCTTGGCTTGGGCCTGGTGATACTGAATTCAGTTCAGG TCATTCCTACACTACTGGTGGTGCGTTAGGCCTTCAGGCAACTGAAAAAGCAGGAACA AATAAAGTGCTTGCAGGAATTGTTTCTTCAAAAGCAACTTTTACATACCCACTTTATCTT GAGGCAATGGTAAAACCGAGTAATAACACTATGGCTAATGGTGTATGGATGTTGAGCT CTGATTCAACTCAGGAAATTGATGCGATGGAGGCATACGGCAGTGATCGTGTAGGGCA AGAGTGGTTTGACCAACGTATGCACGTAAGTCACCATGTTTTTATACGTGAGCCATTTC AAGATTACCAACCAAAAGATGCAGGCTCTTGGGTATACAATAACGGCGAAACATACCG AAATAAATTTCGTCGCTACGGTGTTCATTGGAAGGACGCGTGGAACCTAGATTACTATA TTGATGGTGTATTAGTTCGCAGCGTTTCAGGTCCTAATATAATTGATCCTGAAGGCTAT ACCGGTGGCACAGGGCTAAGTAAACCAATGCACATCCTTTTAGATATGGAACATCAAC CTTGGCGTGATGTAAAACCAAATTCAGCCGAGCTAGCTGATTCAAACAAAAGTATATTT TGGATTGACTGGATACGTGTCTACAAAGCAAACTAAGTCATTCTAAAATATTTGTAATAT TAGGTTTTATTGCTTCTCGTTATACGACACGGAGCAATAAACTTTAAGGTCCCCAAAACT ACTTAATGCGGCTATTACAGCCGCATTAAGTATAATTAACCTGAACTCTGGATAGTAAAT CTATCTCGAGCAGCTATTGACGCGTGAATTCTCTCCCTATAGTGAGTCGTA AG52 agarase full sequence ORF

16. GGGGATTGTAGAGTTCTTTCCCTTAGAAGATTAAAGATGGGGCGGCGACCAGCCCGTTGCTACCGCTACTGTAAA AACAAGCCGTATCCCAAGTCACGGTTTTGCCGTGGTGTCCCAGATCCAAAGATCCGAATCTTTGATTTGGGCAGAA AGAAGGCTCGTGTTGATGAGTTCCCTCTCTGCATCCACTTGATCTCCGATGAGTATGAACAGCTGTCATCGGAAGC TCTGGAGGCTGGACGTATCTGCGCCAACAAGTACCTGGTGAAGGTCTGCGGCAAGGATTCCTTCCACTTACGTGT GCGCTTACATCCCTTCCACGTCATCAGGATCAACAAGATGTTGTCCTGTGCCGGTGCTGATCGACTTCAGACCGGT ATGCGTGGTGCTTGGGGTAAACCTCAAGGGACTGTGGCCCGTGTAAACATTGGACAGCCGATCATGTCGGTCCGT TCCAGAGAGCAGAACGAGTCTGCTGTGATTGAGGCCCTCCGGAGAGCCAAGTTCAAGTACCCTGGCAGACAGAA GATTGTGCTCTCCAAGAAGTGGGGATTCACCAAGTGGCCAAGAGACTGCTATGAGGATATGTGCGCTGATGGACG CCTGATTCCTGACGGTGTCAGTGTTCAGTACAGACCCAACCGAGGCCCTCTCAACAAGTGGAGAAAGGACCAGAC CAACCTCAGGTCCtagACAGACTGTTGGGACGAACATGGTCATGTGAATAAAGAGCTGGAGAAATAAAAAAAGAAAA AAAAAAAAAAAAAAAAAAAAAAAAA 전복 QM protein - full sequence ORF GDCRVLSLRRLKMGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGR KKARVDEFPLCIHLISDEYEQLSSEALEAGRICANKYLVKVCGKDSFHLRVR LHPFHVIRINKMLSCAGADRLQTGMRGAWGKPQGTVARVNIGQPIMSVRS REQNESAVIEALRRAKFKYPGRQKIVLSKKWGFTKWPRDCYEDMCADGR LIPDGVSVQYRPNRGPLNKWRKDQTNLRS

22. ATGGACATTCCCATTACAAAACACTGGGATGGTGGTTTTCGGTCGGACTTCTGTGAACCTATTACT CAAACCATGCATTCCTGGAAAGCGCATGTCATCTTTGACCATCACGTCGATACGCTAGACATCTGG GTTGCTGATGTTCAGCAAACTCTGAATGGAGGTAAAGAGTTTGTCCTGGTCAACAAGGCATCTTAT GGCGAGCAGAAGGCTGGCGACAAACTGTGTGTGAAACTAATTGGACGTGTCAACGGTGACATCGT TCCAAAAGGCCGGTTCTACATTGAAGGCATGGACGGCCCGGTATCAGCTACCGAGAAACCCATCA GACATACATACAAACCGGGCACGCCGACAACGTCTAGCCATGTCACAGGCAAAGTTCTTTATGAAT ATTATGGATTCGATCCGAGTGATTACAAAAAGGGCATAACTGTGCTACAACATGGAGGCTTCGATG AAGACTCTGGCTCCGTTGTCCTTGACCCTGCCGGTACCGGGGAACATGTCCTCAAAGTGTTCTAC GAGAAGGGACACTATATCAAAGTTCGGGGCCACCGTGGGATTCAGTTCTACTGGACCCCTATCCA TCCCCAAACGACGCTGACGTTGAGCTACGACATCTACTTCGACCCCAACTTTGACTGGGTTAAGG GAGGCAAGCTTCCGGGTCTGTGGGGGAGGGTCCCAAACCCTGTTCTGGGGGACGTCACAACGAG GAATGTTTCTCAACACGCTTCATGTGGAGGACTGGTGGGGGTGGGGAACTGTACGCCTACATCCC CTCTGGACAACGCGCCGACTTCTGCACTAAGAACATTTGCAACTTCGACTACGGTAACTCTTtag 전복 alginate lyase - ORF