1. ASSESSMENT OF SOIL HEALTH USING NEMATODE ASSEMBLAGE AND SOIL CHARACTERISTICS IN ARENOSOLS, CAMBISOLS AND VERTISOLS IN KENYA Andrew Thuo Kariuki A56/77793/2012 Supervisors: Prof. J.W. Kimenju Dr. G. Karuku Dr. G.M. Kariuki Prof. H. Melakeberhan

2. Introduction Diminishing Cropland Productivity is threatened by excessive, wanton cultivation and deforestation (Hill et al. 1995b). Cropland accounts for 31% of Kenya’s total area, contributing to 20% of GDP and 65% of Exports. (Republic of Kenya 2010) Soil should be perceived as a “Living Body” hence Soil health quality assessment requires a prompt and direct analyses technique ( Torrion, 2002). Soil micro-organisms depict life processes taking place in soil (Neher, 2001). Soil Nematode Assemblages act as key bio-indicators of Soil life processes. (Neher,2001;Fiscus & Neher, 2002).

3. Introduction (cont..) Nematode Fauna Fluctuations indicate changes in soil ecosystem functions (Kimenju et al. 2009). Past Soil health assessment via nematodes has focused “Primarily” on Plant Parasitic Nematodes (Urzelai et al., 2000). A New and Robust approach to assess entire soil food web via use of :- » Bacteriovores » Fungivores » Herbivores » Predators » Omnivores (Ferris et al.,2005; Wardle et al., 2005). Nematode Trophic Levels

4. Justification -Dwindling Cropland due to soil degradation in Kenya necessitate the Need to optimize on under-utilized available croplands in Kenya. -Nematode Assemblages act as Viable, Rapid and Accurate Tool for Monitoring Soil Health in Kenya due to their: Presence in all Soil Trophic Levels Change Sensitive Reaction (chemical & physical) Differentiation btw Natural & Structural changes Economical and Accessible

5. Objectives General Objective: To develop accurate and scalable soil health-based models that address soil degradation using Vertisols, Arenosols and Cambisols of Kenya. Specific Objectives: 1.To determine effect of seasonal variation and land use practices on nematode assemblage of selected soil groups. 2.To determine how soil properties influence nematode assemblage in selected soil groups. 3. To determine how socio-economic & local customs influence land use practices in selected soil group regions.

6. Methodology Total of 192 Geo-Referenced Samples 2 Farms (24) 2 Farms (24) 2UD Zones (8) 2UD Zones (8) 2 Farms (24) 2UD Zones (8) 2 Farms (24) 2UD Zones (8) 2 Farms (24) 2 Farms (24) 2UD Zones (8) 2UD Zones (8) 2 Farms (24) 2 Farms (24) 2UD Zones (8) 2UD Zones (8) 2 Farms (24) 2 Farms (24) 2UD Zones (8) 2UD Zones (8) Vertisols Cambisols Arenosols Vertisols Cambisols Arenosols Soil Sites Identification (FAO 1974) Soil Sites Identification (FAO 1974) Central Province Sites (Makuyu) Central Province Sites (Makuyu) Lower Eastern Province Sites (Makueni)

7. Methodology…. Seasonal Sampling Schedule Dry Hot Season (192) Dry Hot Season (192) Rainy Wet Season (192) Rainy Wet Season (192) Dry Cold Season (192) Dry Cold Season (192) 1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 8 9 9 10 192 200cm 3 Soil for nematode extraction 200cm 3 Soil Sample for Soil Property Analyses Contingency Soil Sample Compilation of Soil Variables Soil Information Questionnaire Administered- Socio-Economic Survey

8. Description of Lab Data to Collect Nematode Data  Microscopic Genus Level ID (×400)  Trophic Levels  Genus Richness  Abundance Ratio  Maturity Index  Structure Index Soil Properties -Physical Properties (Temperature, Sand%, Silt%, Clay%) -Chemical Properties (pH, Ca, Mg, K, Na, Total N, Available P, Cation Exchange Capacity C.E.C., Organic Matter Content). Socio Economic Survey- Land Use and Socio-economic info

9. Data Analyses Data will be subjected to Analysis of Variance (ANOVA) using SPSS, GenStat & SAS. Differences among the Soil Groups and Regions will be tested using ANOVA for the comparison if the F-value is significant.

10. Expected Output Effect of seasons, soil groups and land use practices on nematode assemblage established Effect of soil properties on nematode assemblage under different soil groups determined Msc Thesis Three scientific papers

11. Work Plan Activities 2012 2013 JuneJulyAugustSepOctNovDecJanFebMarAprMayJuneJuly Soil Identification and 1st Season Sampling Second Season Sampling Socio Economic survey Nematode enumeration Data compilation Data Analyses and thesis Writing Submission

12. Budget ActivityQuantityUnit cost KshTotal cost (Ksh) Data collection expenses / Nematodes Count/Soil Property Analyses 300,000 Subsistence and Travel8200016,000 Printing Papers Thesis2 reams5001,000 Printing Draft Proposal & Final Thesis 200 Pages1010,000 Photocopying400 Pages2800 Binding5 copies50250 GRAND TOTAL 328,050

13. Thank You