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NSF RET Fellowship at UIC RET Teaching Module Bill Farmer – Evanston Township High School University of Illinois, Chicago Advisor: Prof. David Schneeweis Graduate Student Mentor: Sujata Sundara-Rajan REU Students: Amin Farokhrani and Jaime McCoin Summer 2005
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NSF RET Fellowship at UIC Presentation Outline RET Background RET Background Teaching Module Overview Teaching Module Overview Data Analysis Data Analysis Inquiry Lab Inquiry Lab Outcomes and Standards Outcomes and Standards
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NSF RET Fellowship at UIC RET Background The primary objective of my research group was the development of an interface between a small biocompatible microchip and an existing biological system. The primary objective of my research group was the development of an interface between a small biocompatible microchip and an existing biological system. Such technology would be applicable to a design for a retinal prosthesis to restore partial vision to patients who have experienced photoreceptor degeneration. Such technology would be applicable to a design for a retinal prosthesis to restore partial vision to patients who have experienced photoreceptor degeneration.
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NSF RET Fellowship at UIC RET Background Creating such an interface requires the development of an effective and reliable method to guide the growth of human tissue cells, primarily retinal neurons, in such a way so that it establishes a connection between the body and the microchip. Creating such an interface requires the development of an effective and reliable method to guide the growth of human tissue cells, primarily retinal neurons, in such a way so that it establishes a connection between the body and the microchip. Our research group ’ s end goal was to conduct in vitro tests of a soft lithography micropatterning technique with human cell tissue cultures. Our research group ’ s end goal was to conduct in vitro tests of a soft lithography micropatterning technique with human cell tissue cultures.
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NSF RET Fellowship at UIC My Contribution In preparation for the in vitro tests I was responsible for the growth and maintenance of human fibroblast cells. In preparation for the in vitro tests I was responsible for the growth and maintenance of human fibroblast cells. Human fibroblast cells were used initially due to their relative ease in cell culture compared to retinal neuron cells. Human fibroblast cells were used initially due to their relative ease in cell culture compared to retinal neuron cells.
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NSF RET Fellowship at UIC Fibroblast Tissue Culturing Utilization of sterile technique Utilization of sterile technique Cells maintained at 37°C and 5% carbon dioxide Cells maintained at 37°C and 5% carbon dioxide Passage of cells at 75% confluence Passage of cells at 75% confluence 10x Magnification Fibroblast Cells 20x Magnification Fibroblast Cells
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NSF RET Fellowship at UIC Data Acquisition For My Classroom Digital images were acquired to track the growth patterns of the fibroblast cell cultures. Digital images were acquired to track the growth patterns of the fibroblast cell cultures. I set up a controlled experiment where I manipulated the initial concentration of cells and monitored its impact on growth rates. I set up a controlled experiment where I manipulated the initial concentration of cells and monitored its impact on growth rates. Digital images of fixed cells stained with DAPI were also taken for potential use in the classroom. Digital images of fixed cells stained with DAPI were also taken for potential use in the classroom.
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NSF RET Fellowship at UIC Teaching Module Overview In order to incorporate my RET experience into my classroom I designed a two part mini unit for my mixed-level biology classes. In order to incorporate my RET experience into my classroom I designed a two part mini unit for my mixed-level biology classes. The first part of the unit involves the student analysis of the digital fibroblast cell data. The first part of the unit involves the student analysis of the digital fibroblast cell data. The second part of the unit involves the development of an inquiry-based lab where students will design their own controlled experiment to test the impact of a chosen variable on the growth rate of bacteria. The second part of the unit involves the development of an inquiry-based lab where students will design their own controlled experiment to test the impact of a chosen variable on the growth rate of bacteria.
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NSF RET Fellowship at UIC Analysis of Fibroblast Data Students will … Students will … Utilize freeware Scion Image software available through the NIH website to view the images of the human fibroblast cells calculate the cell population and densities. Utilize freeware Scion Image software available through the NIH website to view the images of the human fibroblast cells calculate the cell population and densities. Organize their data in computer generated tables and graphs using Microsoft Excel. Organize their data in computer generated tables and graphs using Microsoft Excel. Analyze the data and draw conclusions with regard to the impact of initial cell concentration on the growth rate of human fibroblast cells. Analyze the data and draw conclusions with regard to the impact of initial cell concentration on the growth rate of human fibroblast cells.
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NSF RET Fellowship at UIC Analysis of Fibroblast Data Sample Excel Graph of Fibroblast Data Sample Excel Graph of Fibroblast Data
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NSF RET Fellowship at UIC Analysis of Fibroblast Data This portion of the module will be conducted through the course of two 43 minute class periods. This portion of the module will be conducted through the course of two 43 minute class periods. The only materials necessary for this module are computers available either in the computer center or class sets of portable laptop go-carts. The only materials necessary for this module are computers available either in the computer center or class sets of portable laptop go-carts. Students will generate a typed formal lab report. Students will generate a typed formal lab report.
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NSF RET Fellowship at UIC Inquiry Lab Students will … Students will … Develop a procedure for a controlled experiment to test how the manipulation of their chosen variable impacts the rate of bacterial growth. Develop a procedure for a controlled experiment to test how the manipulation of their chosen variable impacts the rate of bacterial growth. Learn about sterile technique and how to culture bacteria cells. Learn about sterile technique and how to culture bacteria cells. Conduct their designed experiments and gather data on bacterial growth patterns. Conduct their designed experiments and gather data on bacterial growth patterns. Organize and analyze their data in a formal lab report write-up. Organize and analyze their data in a formal lab report write-up.
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NSF RET Fellowship at UIC Inquiry Lab This portion of the module will be conducted through the course of 4 days. This includes two 43 minute single periods and two 86 minute double periods. This portion of the module will be conducted through the course of 4 days. This includes two 43 minute single periods and two 86 minute double periods. Two used incubators have been acquired from other teachers within my department which will enable my class to culture bacteria. Learn about sterile technique and how to culture bacteria cells. Two used incubators have been acquired from other teachers within my department which will enable my class to culture bacteria. Learn about sterile technique and how to culture bacteria cells. The remaining equipment necessary for culturing bacteria is available within the school ’ s supplies. The remaining equipment necessary for culturing bacteria is available within the school ’ s supplies.
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NSF RET Fellowship at UIC ETHS Standards 19. Formulate testable hypotheses. 19. Formulate testable hypotheses. 20. Design and conduct scientific investigations by identifying and clarifying the question, method, controls and variables; organizing and displaying data; and explaining the results. 20. Design and conduct scientific investigations by identifying and clarifying the question, method, controls and variables; organizing and displaying data; and explaining the results. 21. Know scientific explanations or models must be: logically consistent supported by evidence, open to questions and possible modifications, and based on historical and current scientific knowledge. 21. Know scientific explanations or models must be: logically consistent supported by evidence, open to questions and possible modifications, and based on historical and current scientific knowledge. 22. Use technology and math to improve investigations and communications; use computers for the collection, analysis, and display of data. 22. Use technology and math to improve investigations and communications; use computers for the collection, analysis, and display of data.
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NSF RET Fellowship at UIC Illinois Science Standards 11.A.4a Formulate hypotheses referencing prior research and knowledge. 11.A.4a Formulate hypotheses referencing prior research and knowledge. 11.A.4b Conduct controlled experiments or simulations to test hypotheses. 11.A.4b Conduct controlled experiments or simulations to test hypotheses. 11.A.4c Collect, organize and analyze data accurately and precisely. 11.A.4c Collect, organize and analyze data accurately and precisely. 11.A.4d Apply statistical methods to the data to reach and support conclusions. 11.A.4d Apply statistical methods to the data to reach and support conclusions. 11.A.4e Formulate alternative hypotheses to explain unexpected results. 11.A.4e Formulate alternative hypotheses to explain unexpected results. 11.A.4f Using available technology, report, display and defend to an audience conclusions drawn from investigations. 11.A.4f Using available technology, report, display and defend to an audience conclusions drawn from investigations.
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NSF RET Fellowship at UIC Questions? Questions Welcome
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NSF RET Fellowship at UIC Acknowledgements National Science Foundation National Science Foundation University of Illinois, Chicago University of Illinois, Chicago Prof. Andreas Linninger - Director Prof. Andreas Linninger - Director Prof. Christos Takoudis – Co-Director Prof. Christos Takoudis – Co-Director Prof. David Schneeweis - Advisor Prof. David Schneeweis - Advisor Amin Farokhrani and Jaime McCoin – REU Students Amin Farokhrani and Jaime McCoin – REU Students
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NSF RET Fellowship at UIC References 1. H Kolb. How the retina works. American Scientist, Jan.-Feb. 2003. 1. H Kolb. How the retina works. American Scientist, Jan.-Feb. 2003. 2. J. Chang et al. A modified microstamping technique enhances polylysine transfer and neuronal cell patterning. Journal of Biomaterials, 2003. 2. J. Chang et al. A modified microstamping technique enhances polylysine transfer and neuronal cell patterning. Journal of Biomaterials, 2003. 3. D. Branch et al. Long-term stability of grafted polyethylene glycol surgaces for use with microstamped substrates in neuronal cell culture. Journal of Bimaterials, 2001. 3. D. Branch et al. Long-term stability of grafted polyethylene glycol surgaces for use with microstamped substrates in neuronal cell culture. Journal of Bimaterials, 2001. 4. D. Branch et al. Long-term maintenance of patterns of hippocampal 4. D. Branch et al. Long-term maintenance of patterns of hippocampal pyramidal cells on substrates of polyethylene glycol and microstamped polylysine. IEEE Transactions on Biomedical Engineering, March 2000. pyramidal cells on substrates of polyethylene glycol and microstamped polylysine. IEEE Transactions on Biomedical Engineering, March 2000. 5. A. Bernard et al. Microcontact printing of proteins. Journal of Advanced Materials, July 2000. 5. A. Bernard et al. Microcontact printing of proteins. Journal of Advanced Materials, July 2000. 6. B.Wheeler et al. Microcontact printing for precise control of nerve cell growth in culture. Journal of Biomechanical Engineering, February 1999. 6. B.Wheeler et al. Microcontact printing for precise control of nerve cell growth in culture. Journal of Biomechanical Engineering, February 1999. 7. R. Kane et. al. Patterning proteins and cells using soft lithography. Journal of Biomaterials, 1999. 7. R. Kane et. al. Patterning proteins and cells using soft lithography. Journal of Biomaterials, 1999. 8. D. Branch et al. Microstamp patterns of biomolecules for high-resolution neuronal networks. Medical & Biological Engineering & Computing, 1998. 8. D. Branch et al. Microstamp patterns of biomolecules for high-resolution neuronal networks. Medical & Biological Engineering & Computing, 1998. 9. B. Ujhelyi and K. Garsha, Patterned growth of neurons in vitro. Beckman Institute for Advanced Science and Technology, UIUC, 2003. 9. B. Ujhelyi and K. Garsha, Patterned growth of neurons in vitro. Beckman Institute for Advanced Science and Technology, UIUC, 2003. 10. B. Wheeler et al., http://soma.npa.uiuc.edu/labs/wheeler/home.html. UIUC, 2005. 10. B. Wheeler et al., http://soma.npa.uiuc.edu/labs/wheeler/home.html. UIUC, 2005.http://soma.npa.uiuc.edu/labs/wheeler/home.html
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