1. InfraCal Ethanol Blend Analyzer Ethanol in Gasoline Calibration for High Levels www.WilksIR.com

2. Calibration Standards Calibration Standards are available from Wilks Enterprise or you can make your own

3. Mix at least 5 standards within the expected measurement range Mix at least 5 standards within the expected measurement range A maximum of 20 calibration points can be entered in the calibration table A maximum of 20 calibration points can be entered in the calibration table Calibration Standards

4. Reference for Mixing Volumetric Standards % Gasoline Ethanol 519 ml1 ml 1018 ml2 ml 1517 ml3 ml 2016 ml4 ml 2515 ml5 ml 3014 ml6 ml 3513 ml7 ml 4012 ml8 ml 4511 ml9 ml 5010 ml10 ml % Gasoline Ethanol 559 ml11 ml 608 ml12 ml 657 ml13 ml 706 ml14 ml 755 ml15 ml 804 ml16 ml 853 ml17 ml 902 ml18 ml 951 ml19 ml 100020 ml

5. Clean the crystal with a delicate task wipe cleaning from one end of the crystal to the other Background Measurement

6. Press and hold the ZERO button until the display reads bAL Recall ZERO CAL RUN bAL Background Measurement

7. Recall ZERO CAL RUN 1.267 A multiplier value to 3 decimal places will be displayed when zero is established The actual value is only of interest when reporting problems to the factory Background Measurement

8. Recall ZERO CAL RUN 0.0 Background Measurement Press RUN The display should read 0.0 + 0.2 If not, repeat the zero process

9. Run each standard at least 3 times Average results for each standard Enter results in a calibration table in column “A” and the corresponding calibration value in column “C” (see next slide) Calibration with Prepared Standards

10. AAbsorbanceC Calibration Standard AO1= 90.1 CO1= 70.0 AO2= 92.6 CO2= 80.0 AO3= 94.2 AO3= 94.2 CO3= 90.0 CO3= 90.0 Example Calibration Table

11. Entering Values into EDIT program Make sure the Cal Lock switch on the back ofMake sure the Cal Lock switch on the back of the InfraCal is in the “I” position the InfraCal is in the “I” position

12. Press the CAL button for two seconds, until CALPress the CAL button for two seconds, until CAL appears on the display appears on the display Recall ZERO CAL RUN CAL Entering Values into EDIT program

13. Recall ZERO CAL RUN Edit Press RECALL until Edit is displayedPress RECALL until Edit is displayed Entering Values into EDIT program

14. Recall ZERO CAL RUN n= Press CAL The display will read n= for a short time Entering Values into EDIT program

15. Recall ZERO CAL RUN 3 Set the number to the number of standards (for our example it will be 3) using the up and down arrowsSet the number to the number of standards (for our example it will be 3) using the up and down arrows Entering Values into EDIT program

16. Recall ZERO CAL RUN A01 Press CAL and the display will read A01= for a short timePress CAL and the display will read A01= for a short time Entering Values into EDIT program

17. Using the up and down arrows, scroll to the first absorbance value (for our example it will be 90.1)Using the up and down arrows, scroll to the first absorbance value (for our example it will be 90.1) Entering Values into EDIT program AAbsorbanceC Calibration Standard AO1= 90.1 CO1= 70.0 AO2= 92.6 CO2= 80.0 AO3= 94.2 AO3= 94.2 CO3= 90.0 CO3= 90.0

18. Recall ZERO CAL RUN 90.1 Using the up and down arrows, scroll to the first absorbance value (for our example it will be 90.1)Using the up and down arrows, scroll to the first absorbance value (for our example it will be 90.1) Entering Values into EDIT program

19. Recall ZERO CAL RUN C01 Press CAL again and the display will read C01=Press CAL again and the display will read C01= Entering Values into EDIT program

20. Using the up and down arrows, scroll to the first calibration value (for our example it will be 70.0)Using the up and down arrows, scroll to the first calibration value (for our example it will be 70.0) Entering Values into EDIT program AAbsorbanceC Calibration Standard AO1= 90.1 CO1= 70.0 AO2= 92.6 CO2= 80.0 AO3= 94.2 AO3= 94.2 CO3= 90.0 CO3= 90.0

21. Recall ZERO CAL RUN 70.0 Using the up and down arrows, scroll to the first absorbance value (for our example it will be 70.0)Using the up and down arrows, scroll to the first absorbance value (for our example it will be 70.0) Entering Values into EDIT program

22. Recall ZERO CAL RUN A02 Press CAL again and the display will read A02=Press CAL again and the display will read A02= Entering Values into EDIT program

23. Using the up and down arrows, scroll to the second absorbance value (for our example it will be 92.6)Using the up and down arrows, scroll to the second absorbance value (for our example it will be 92.6) Entering Values into EDIT program AAbsorbanceC Calibration Standard AO1= 90.1 CO1= 70.0 AO2= 92.6 CO2= 80.0 AO3= 94.2 AO3= 94.2 CO3= 90.0 CO3= 90.0

24. Recall ZERO CAL RUN C02 Press CAL again and the display will read C02=Press CAL again and the display will read C02= Entering Values into EDIT program

25. Using the up and down arrows, scroll to the second calibration value (for our example it will be 80.0)Using the up and down arrows, scroll to the second calibration value (for our example it will be 80.0) Entering Values into EDIT program AAbsorbanceC Calibration Standard AO1= 90.1 CO1= 70.0 AO2= 92.6 CO2= 80.0 AO3= 94.2 AO3= 94.2 CO3= 90.0 CO3= 90.0

26. Do the same for the remaining calibration valuesDo the same for the remaining calibration values Entering Values into EDIT program AAbsorbanceC Calibration Standard AO1= 90.1 CO1= 70.0 AO2= 92.6 CO2= 80.0 AO3= 94.2 AO3= 94.2 CO3= 90.0 CO3= 90.0

27. Recall ZERO CAL RUN IDLE After the last value, the display will read IDLEAfter the last value, the display will read IDLE Entering Values into EDIT program The calibration is now stored and active in the InfraCal

28. First insure sample plate is clean Sample Analysis

29. Apply approximately 2ml of the fuel sample ensuring that the entire crystal is wet Sample Analysis

30. Close cover to keep volatiles from evaporating Sample Analysis

31. Recall ZERO CAL RUN 0.0 Press RUN Sample Analysis

32. Recall ZERO CAL RUN 85.7 Sample Analysis After 15 seconds the result is displayed

33. Clean the crystal with a delicate task wipe cleaning from one end of the crystal to the other The InfraCal is ready for the next sample Sample Analysis

34. InfraCal Ethanol Blend Analyzer Ethanol in Gasoline Calibration for High Levels For questions please contact us at: tech@WilksIR.com, or 203-855-9136