1. Max Showalter 7. June 2012 Purdue University

2. Let’s begin at the parts registry homepageparts registry Click On When you get to this webpage, you will see several different icons you can click on.

3.  The first thing you’ll notice on this page is a long list of Promoters, Ribosome Binding Sites, etc…  Further down, you will see that you can browse parts by part, type, function, or chassis From this page and the search bar in the top right your job will be to find the pieces for a functional device.

4.  If I’m going to request a part for my iGEM Team…  What email address do I send my request to?  What do I include?

5.  If I make a BioBrick part:  What will the suffix be?  What will the suffix be if I use Spe1 to cut my plasmid?  What temperature should I do my digestion if I am using Spe1?

6.  Find the part(s) that stopped transcription in the banana smell generating device (You will have to find the banana smell generating device first!)  What are these parts called?  What are their part registry numbers?  How many base pairs does each have?

7.  Find a constitutive eukaryote promoter that works and has a registry star  Find a Kozak sequence for yeast that works and has a registry star  A protein that will make your yeast fluoresce cyan – [Cyan Fluorescent Protein]  A terminator that works in yeast (What is the name of the Chassis for these

8. All Biological Circuits must have: 1. Promoter 2. Ribosome Binding Site (RBS) 3. Coding Region 4. Terminator Rules to the Game Use Only: 1.Parts that work 2.Parts that are available Specify a chassis and ensure your parts match this chassis Now It’s Time to Assemble a Part

9. Let’s Make a Metal Sensor.. Metal Ions Fe Device

10.  Assemble a list of the parts required for the device  Follow all the rules given

11. Team A Develop two systems for E. coli that will: 1. Make the bacteria fluoresce cyan 2. Make the bacteria smell like winter mint in the presence of salicylic acid Team B Develop two different systems for S. cerevisiae that will 1.Make the yeast fluoresce Yellow 2.Make the yeast fluoresce orange As a bonus: yeast (a eukaryote) doesn’t use a ribosome binding site in the same sense as prokaryotes. What is the analogous part in yeast? Describe how it is like an RBS.

12.  Make a double tiered system that will turn off color when banana smell has been turned on