1. • TEMPO® Concept • Workflow
• bioMerieux
• TEMPO® Concept • Workflow
• Test Menu
• Official Validations
Jamaica, NY
5/30/2008
Philippe Villard, Business Development Manager, Quality Indicators

2. • TEMPO® Concept • Workflow
• bioMerieux
• TEMPO® Concept • Workflow
• Test Menu
• Official Validations
Philippe Villard, Business Development Manager, Quality Indicators

3. bioMérieux develops, produces and markets solutions composed of: Instruments, Reagents & Software.
• Clinical Diagnostic
• Industrial Diagnostic (Food, Pharmaceutical & Cosmetic)

4. Research & Development
Lyon : Marcy, Craponne, La Balme
(France)
Boxtel
(Netherlands)
Saint Louis
Grenoble
(USA)
(France)
Durham
(USA)
Sao Paulo
(Brazil)
Florence
(Italy)
OVER 850
12.5% OF
400 FAMILIES
9 R&D SITES
EMPLOYEES
SALES
OF PATENTS
INVESTED

5. bioMerieux Solutions Culture Media
Manual Identification BioBallTM for Quantitative QC

6. bioMerieux Solutions Vitek 2 Compact® for bacterial Enumeration
diversilabTM for genotyping

7. bioMerieux Solutions VIDAS® for Pathogen Detection
BacT/Alert® for Spoilage Organism Detection
TEMPO® for Quality Indicator Quantification

8. • TEMPO® Concept • Workflow
• bioMerieux
• TEMPO® Concept • Workflow
• Test Menu
• Official Validations
Philippe Villard, Business Development Manager, Quality Indicators

9. From the Traditional Approach
1 ml
1 ml
1 ml +
90 ml
10 g of product
10-1
10-2
10-3
10-4
I n c u b a t i o n
CFU/ g
Interpretation
3 +
2 +
2 +
acc to statistical
3/2/2
Counting
table

10. To an Innovative Solution

11. TEMPO Protocol Automated Reading + Diluent
Sample + dehydrated specific culture media Automated Filling of the card
Standard Incubation
Automated Reading
3,7 Log count
Automated discrimination of positive and negative wells
Enumeration range !
Most Probable Number computing
2 / 0 / 0
Statistic table + dilution factor Enumeration (cfu/g)

12. Bacterial Growth Detection
non fluorescent
fluorescent
Total Viable Count
E.coli
4MU-X
4MU
Bacterial specific enzymatic
activity
Media acidification due to carbohydrate metabolism
Enterobacteriaceae
Coliforms
4MU
4MU
fluorescent
non fluorescent

13. Enumeration Range MPN 16 tubes = 3.7 log of range 1 10 -1 102 -2 103
Sample 1
4.9x103 cfu/g
Dilution
3.7 log 10
4.9x104 cfu/g -1
102
4.9x105 cfu/g -2
103
4.9x106 cfu/g -3
104
4.9x107 cfu/g -4

14. TEMPO TVC 102 - 4.9.105 cfu/g TEMPO CC 10 - 4.9.104 cfu/g TEMPO EC
0.1mL
cfu/g
1mL
TEMPO CC
cfu/g
Diluted Sample
1mL
TEMPO EC
cfu/g
No dilution tubes (reduce turn around time, reduce testing bias) Standardized Preparation, Reading & Recording of the tests Improves accuracy and reproducibility of the tests

15. • TEMPO® Concept • Workflow
• bioMerieux
• TEMPO® Concept • Workflow
• Test Menu
• Official Validations
Philippe Villard, Business Development Manager, Quality Indicators

16. Tomorrow with TEMPO® Today… 2- Sample preparation 1- Media preparation
3- Dilution
4-Inoculation
5- Filling
8-Reporting
7-Reading
6-Incubation

17. TEMPO Workflow (Video)
Sample preparation
Inoculation
Incubation
Reading & interpretation

18. TEMPO® Test Results Trending Analysis
Sample ID Test Dilution (1/) Preparation Date Status CFU/G Annotations Comments
05\3382
TVC
40000
lun. 21/03/ :23 OK =
05\3382 CC 40
lun. 21/03/ :23 OK
= 4800
05\3382 EC 40
lun. 21/03/ :24 OK
< 10
05\3398
TVC
4000
lun. 21/03/ :27 OK =
05\3398 CC 40
lun. 21/03/ :28 OK
= 930
05\3398 EC 40
lun. 21/03/ :28 OK
< 10
05\3617
TVC
4000
lun. 21/03/ :32 OK
>
05\3617 CC 40
lun. 21/03/ :32 OK
= 1800
05\3617 EC 40
lun. 21/03/ :33 OK
= 10
05\3618
TVC
4000
lun. 21/03/ :38 OK
= 25000
05\3618 CC 40
lun. 21/03/ :38 OK
= 10
05\3618 EC 40
lun. 21/03/ :45 OK
< 10
05\3621
TVC
4000
lun. 21/03/ :47 OK =
05\3621 CC 40
lun. 21/03/ :48 OK
= 240
Trending Analysis

19. TEMPO® - Time Saving = Resource Allocation
Based on external independent studies :
Tempo saves from 25 to 80% labor time!
Labor hours required per steps and per day
In hours
4.5
Plate Method QI
3.0
-70 samples
2.5
-200 QI
tests/day
2.0
1.5
0.0
Sample Preparation
Media preparation
Tubes preparation Identification/ Dilution/
Reading and
Washing if necessary
Inoculation / Filling
Recording

20. TEMPO : Throughput 500 tests prepared in 1 day by 1 operator
500 tests read & reported in < 2 hours by 1 operator

21. • TEMPO® Concept • Workflow
• bioMerieux
• TEMPO® Concept • Workflow
• Test Menu
• Official Validations
Philippe Villard, Business Development Manager, Quality Indicators

22. TEMPO test kits Available Total Viable Count Enterobacteriaceae
Coliform Count
Generic E.coli
• Current Developments • Staphylococcus aureus
• Launch July 2008
• Lactic Acid Bacteria
• Launch July 2008
• Yeasts & Molds
• Launch 1st Quarter 2009

23. • TEMPO® Concept • Workflow
• bioMerieux
• TEMPO® Concept • Workflow
• Test Menu
• Official Validations
Philippe Villard, Business Development Manager, Quality Indicators

24. Continuous Official & International Approvals
AOAC Research Institute
• Total Viable Count • Enterobacteriaceae • Coliforms
• E.coli
AOAC OMA
• Total Count: Approval Pending • E.coli & Coliforms: In progress
ISO 16140
• Total Viable Count • Enterobacteriaceae • Coliforms
• E.coli

25. Thank you
Philippe Villard, Business Development Manager, Quality Indicators