1. Clownfish Breeding Allison Burns and Grace Jackson

2. Kingdom: Animalia Phylum: Chordata Class: Actinopterygii Order: Perciformes Family: Pomacentridae Taxonomy http://a-z-animals.com/animals/clown-fish/

3. Protandrous Hermaphrodites http://www.glassreef.com/livestock_fish.htm

4. Paired Clownfish https://www.google.com/search?q=clownfish

5. Symbiotic Relationship http://www.slideshare.net/firasaljader/clown-fish

6. Ideal Tank Setting

7. Reproductive Track

8. Four Pairs in the Lovett Aquarium 1.Tank A: Premnas cf. biaculeatus 2. Tank 10: Amphiprion bicintus 3. Tank 11:Amphiprion ocellaris 4. Tank B: Premnas cf. biaculeatus

9. Hypothesis 1.Placing a clay pot in Tank A, Tank 10 and the Georgia Aquarium tank, the pairs will be more likely to lay eggs on clay pots than a rock because the pot is simple and does not require a lot of work to maintain. 2.Using a Vossen Larval trap, the larvae will swim to the light because they are phototropic and will then be able to get captured by a Vossen Larval Trap 3.The eggs will become fully developed once the water flow and the temperature of the tank are kept at a specific water quality

10. 1.What can we do to encourage breeding? 2.How can we keep the fry alive? 3.Why are the pairs not mating? Questions

11. 1.Tank A: Premnas cf. biaculeatus

12. -Fed Twice a day -Location Changes Made to Encourage Breeding

13. 2. Tank 10: Amphiprion bicintus

14. -Anemones -Flower Pots -Fed Twice a day Changes Made to Encourage Breeding

15. 2. Tank 10: Amphiprion bicintus

16. 3. Tank 11: Amphiprion ocellaris

17. Clutch 1: September 24, 2014

18. November 11, 2014 Clutch 2

19. Clutch 2: Video

20. -December 1, 2014 Flower pot Changes Made after Clutch 2

21. April 17th, 2015 Clutch 3

22. April 29th, 2015 Clutch 4

23. Clutch 4: Video

24. 4. Tank B: Premnas cf. biaculeatus

25. Clutch 1:

26. Clutch 2:

27. Clutch 2: Video of Fry

28. Clutch 3:

29. -Feeding twice a day -Temperature change Encouragement Techniques

30. Stable food chain 1.Algae → Rotifers → Larvae Taking Care of Fry

31. -Constant feeding -Setting up a small tank within the tank

32. -Starting algae and rotifer cultures earlier -Consistently feeding twice a day -Recording the differences when feeding the fish twice a day -Finding the most effective way to raise fry Future Directions

33. http://www.reefcentral.com/forums/showthread.php?t=215098 "Aqua Medic Fish Trap." Bulk Free Supply. N.p., n.d. Web. 16 Oct. 2014..http://www.bulkreefsupply.com/aqua-medic-fish- trap.html Hoff, Frank H. Conditioning, Spawning And Rearing Of Fish With Emphasis On Marine Clownfish. N.p.: Florida Aqua Farms, 2009. Print. How To Catch Larvae.MTS. YouTube. N.p., n.d. Web. 16 Oct. 2014..https://www.youtube.com/watch?v=JlbZ2VCLYO0 How To Make A Fry Trap. YouTube. N.p., n.d. Web. 16 Oct. 2014..https://www.youtube.com/watch?v=tVwX_kE6dCA Lanman, Hennie. Breeding Clown Fish Part 1-3. N.p.: n.p., 2002. Print. Lukan, Elizabeth M., ed. Animal-World. N.p., n.d. Web. 16 Oct. 2014..http://animal- world.com/encyclo/marine/clowns/information/breeding-clown-fish-part-two.php Michael, Scott W. "Clownfish Breeding." FishChannel.com. N.p., n.d. Web. 16 Oct. 2014..http://www.fishchannel.com/saltwater-aquariums/species-info/clownfish/breeding-clownfish.aspx Stratton, Richard F. Clownfishes. Neptune City: T.F.H. Publications, 2000. Print. "TangsFire." Amazon.com. N.p., n.d. Web. 16 Oct. 2014..http://www.amazon.com/TangsFire%C2%AE-WF-502B-Fishing- tactical-Flashlight/dp/B00FOILU7A/ref=sr_1_2?s=hi&ie=UTF8&qid=1413438701&sr=1- 2&keywords=flashlight+with+red+filter "Vossen Larval Trap." Etsy. N.p., n.d. Web. 16 Oct. 2014..https://www.etsy.com/listing/117580460/vossen-larval-trap Wittenrich, Matthew L. Breeder's Guide. Neptune City: T.F.H. Publications, 2007. Print. http://www.fishbase.org/summary/11837 Work Cited