1. Microbial Detection Arrays Interim Review I February 6 th, 2007 Aerospace Senior Projects University of Colorado – Boulder Advisors: Dr. Forbes and Dr. Maslanik Customers: BioServe and Tufts University Dave Miller Elizabeth Newton Ted Schumacher Shayla Stewart Steven To Charles Vaughan Sameera Wijesinghe

2. 2 Briefing Overview Overall Design Summary Changes Since CDR Test Plans Schedule Budget Current Issues

3. 3 Overall Design Summary Autoclaves Reagent Water Chamber Peristaltic Pump Mounts Top Shelf Environmental Chamber Reaction Chambers (with Mixers) Mixer Motor Mounts NOTE: Only shows parts to be manufactured

4. 4 Changes Since CDR Mechanical: –Autoclave: Repositioning of screw holes and O-ring groove –Mixer: Rearrangement of impellers, addition of bottom scoop –Environmental chamber: Walls broken up to simplify manufacturing and assembly –Shelves and supports: Sizes standardized, COTS supports Electrical: –Sensors: LM34 temperature sensors on autoclave, reagent water chamber, and ambient PP-0079 thermistors on reaction chamber Additional thermistors placed on reaction chambers Software: –Laptop hard drive for data storage Manufacturing: –BioServe manufacturing parts of autoclave bodies (milling), reaction chamber bodies, and mixers

5. 5 Changes Since CDR Mixer Electrical System Rectangular Mixing Blades Flat Scoop Four Controlled Items

6. 6 Test Plan Flow Chart Power Supply Circuit Switch Board Circuit Peristaltic Pump Control Circuit Autoclave Heater Mixer Control Circuit TEC Control Circuit Integrated Electrical Subsystem Software Input/Output Voltage Integrated System Verification Autoclave Temp/Pressure Sample Transport Mixing Capability Reagent H 2 O Containment Rxn Chamber Environ. Control Autoclave Kill Test Sample Sterility Disassembly/ Reassembly Electrical Mechanical Biological Software Integrated

7. 7 Special Test Requirements Hazard Analyses –Electrical: electrocution/fire –Autoclave: pressure vessel (projectiles), hot surfaces, sharp edges –Assembly/Disassembly: sharp edges –Environmental control: hot surfaces –Integrated System: all of above Testing Locations –BioServe Environmental Chamber –BioServe Wet Chemistry Lab

8. 8 Schedule

9. 9 Budget ExpectedActual Electronics$0$0 (donated) Sensors$0$0 (donated) Mechanical$1,328.53$1,909.61 Computer/DAQ$348.00$606.00 Miscellaneous$150.00$328.01 Total$1,826.53$2,843.62

10. 10 Current Issues Tubing: –PharMed tubing does not come in 1” inner diameter Substitution has been found (Bioprene) but has not been ordered TEC Controller: –Power cord did not come with TEC controller Replacement can be fabricated or purchased Manufacturing: –Two weeks behind schedule Offset by manufacturing contributions from BioServe Ordering: –Pumps, thermostats, and TECs have not been ordered

11. 11 Conclusions Manufacturing behind schedule –Offset by BioServe’s assistance Most parts are ordered –Tubing is only ordering concern –Can be offset by finding other distributors Still on budget –Over what was expected, but still under budget with most parts ordered Mechanical, software, and electrical packages approved Will be able to complete manufacturing and testing by deadlines