1. The present study focuses on the chemical and biological analysis of hydro-alcoholic extracts from the fruits, leaves and branches of Amorpha fruticosa L. (false indigo bush), Fabaceae family. The plant material was collected in October-November 2011 from the outskirts of Iasi and air-dried. Extracts were prepared by percolation with 70% ethanol on a 1:5 drug to solvent ratio. The free radical scavenging activity of the extracts was measured by the DPPH method and the content of total phenolic compounds was assessed by Folin-Ciocalteu colorimetric method. Twelve polyphenolic compounds have been studied in Amorpha fruticosa extracts by HPLC-MS. Antimicrobial activity was evaluated by agar disc diffusion method. Bianca Ivănescu 1, Cristina Lungu 1, Laurian Vlase 2, Cristina Tuchiluş 3, Andreia Corciovă 4 1 Department of Pharmaceutical Botany, Faculty of Pharmacy, UMF “Grigore T. Popa”, Iaşi 2 Faculty of Pharmacy, UMF ”Iuliu Haieganu”, Cluj-Napoca 3 Department of Mycrobiology, Faculty of Pharmacy, UMF “Grigore T. Popa”, Iaşi 5 Department of Drug Analysis, Faculty of Pharmacy, UMF “Grigore T. Popa”, Iaşi Fig. 1. Total phenolic content (mg/g) in Amorpha extracts Table 1. Polyphenol compounds in Amorpha fruticosa extracts nt – not tested a values are mean ± SD of three determinations OBJECTIVES MATERIALS AND METHODS RESULTS CONCLUSIONS REFERENCES 1.Kim YS, Ryu BY et al. Food Chem Toxicol. 2011; 49:1849–1856. 2.Zheleva-Dimitrova DZ. Pharmacogn Mag. 2013; 9(34):109-113. 3.Dat, NT, Lee, JH et al. J. Nat. Prod. 2008;71(10):1696–1700. 4.Borchardt JR, Wyse DL et al. J Med Plants Res. 2008; 2(4):081-093. 5.Diao YP, Li K et al. Chinese Chem Lett. 2009; 20:942–944. 1.Kim YS, Ryu BY et al. Food Chem Toxicol. 2011; 49:1849–1856. 2.Zheleva-Dimitrova DZ. Pharmacogn Mag. 2013; 9(34):109-113. 3.Dat, NT, Lee, JH et al. J. Nat. Prod. 2008;71(10):1696–1700. 4.Borchardt JR, Wyse DL et al. J Med Plants Res. 2008; 2(4):081-093. 5.Diao YP, Li K et al. Chinese Chem Lett. 2009; 20:942–944. BIOLOGICAL AND CHEMICAL RESEARCHES ON AMORPHA FRUTICOSA L. SPECIES Poliphenols Amorpha extract branchesleavesfruits UVMS Conc. mg/gUVMSConc. mg/gUVMS Conc. mg/g Gentisic acid-*--*--*- Caffeic acid-*--*--*- Chlorogenic acid-*--*--*- p-coumaric acid**0.0232**-**0.0557 Ferulic acid**0.0081**0.0121**0.0233 Hyperoside**-**0.2402**0.0122 Isoquercitrin**-**9.4476**0.1765 Rutoside**0.5931**11.8603**0.2754 Quercitrin**0.0117**0.3139--- Quercetin**0.0089**0.0641--- Luteolin**0.0162**0.0093**0.0688 Kaempferol---**0.0202**0.1595 Amorpha fruticosa extracts / Positive controlEC 50 a ( µg/mL) Leaves extract38.03 ± 0.75 Fruits extract18.8 ± 0.2 Branches extract221.16 ± 1.7 Quercetin2.2 ± 0.0 Microorganism Diameter of inhibition zone (mm) Branches extract Leaves extract Fruits extract Ampicillin (25 µg/disc) Chloram phenicol (30 µg/disc) Nystatin (100 µg/disc) Staphylococcus aureus ATCC 259231621192624nt Sarcina lutea ATCC 93411522252826nt Bacillus cereus ATCC 145790016021nt Bacillus subtilis00172629nt Escherichia coli ATCC 259220002129nt Pseudomonas aeruginosa ATCC 27853000016nt Candida albicans ATCC 10231000nt 18 Candida glabrata ATCC MYA 2950000nt 19 Candida sake000nt 20 Table 3. DPPH radical scavenging activities of Amorpha fruticosa extracts The content of total phenolic compounds was highest in leaves extract followed by fruits extract and branches extract. The results of HPLC analysis indicated the presence of ferulic acid, luteolin and rutoside in all samples. The highest scavenging effect was observed in Amorpha fruticosa fruits extract, followed by leaves extract and branches extract. The tested samples, especially the fruits extracts, inhibited the growth of gram positive bacteria. The content of polyphenolic compounds within extracts is correlated with their antioxidant and antimicrobial activity. Table 2. Antimicrobial activity of Amorpha fruticosa extracts