1. Revision – Concept map

2. Human Genome Project Procedures Techniques Genetic mapping
Physical mapping
DNA sequencing
Techniques
Use of microsatellites (STRs)
Restriction enzyme mapping
Sanger method
PCR
Gel electrophoresis

3. Human Genome project Terminology Restriction mapping Primer
Microsatellite / Short Tandem Repeat
Anneal
Genetic marker
Hybridise

4. Detecting Genetic Disorders
Procedures
Pedigree analysis
Linkage analysis (both genetic and physical)
Screening
Techniques
PCR
Chromosome walking
Sanger method
Gel electrophoresis

5. Detecting genetic disorders
Terminology
Probe
Genomic library
Chromosome walking
Hybridise

6. DNA profiling Procedures Techniques DNA isolation
Restriction enzyme digestion
Separation of VNTR
Fixing pattern on filter
Techniques
Gel electrophoresis
Southern blotting

7. DNA profiling Terminology
Minisatellite / Variable Number Tandem Repeat
Probe
Hybridise