1. Planetary Boundaries

10. Dow TNC Collaboration

13. Dow Texas Operations: Non attainment zone for ozone pollution Ozone forms from precursor pollutants: NO x, VOCs Prospect of tightened standards in future  Emissions Abatement through Reforestation?

14. Dow TNC Collaboration 1000 acre project $470,000 122-202 tons NO x removed over 30 years Cost competitive and additional benefits: Other health effects Habitat creation Carbon credits

15. Dow TNC Collaboration Analysis: What Is the Value of Water — for Today and Tomorrow? So how can companies such as Dow account for the value of water and the role that marshes, forests and other ecosystems play in maintaining water flow? And how can those companies use this understanding to plan for current and future availability of water from the Brazos to both its operations and stakeholders in the area — including nature?

16. Dow TNC Collaboration Analysis: What Is the Value of Water — for Today and Tomorrow? -Brazos River demand exceeds supply for 10 to 20% of year; Demand will increase over 50% in next 50 years -Used demand, climate change model to predict water availability; synergistic effects apparent -Identifying "green" and "gray" infrastructure options that could help maintain flow in the river; and -Analyzing the economics of those options to understand which ones will produce the most value for water users, including Dow, and the environment.

17. Dow TNC Collaboration Strategy:

20. Intersex HermesAphrodite Hermaphrodite

21. Dissection and Histology OvaryTestis

22. Dissection and Histology

24. Frequency of Intersex in Green Frogs

25. Prevalence of ‘Intersex Ponds’ CategoryIntersex in : Undeveloped 0 of 2 Ponds Agriculture 4 of 11 Suburban 5 of 6 Urban 3 of 4

26. Focus on the Suburbs: The Ponds of Avon Follow Up Study Using 28 Ponds in One Suburban Town: Intersex Incidence:18% of Individuals Intersex Prevalence:94% of Ponds Caffeine:71% of Ponds

27. Next Step: What’s in the water? Larry Barber, USGS Geoff Giller, Yale Max Lambert, Yale

29. Water Sampling 6 bottles (three each, surface and groundwater) Field Measurements – Conductivity, TDS, DO, Temperature, Depth

30. Frog Collection Green metamorphs 20 - 50 per pond

31. Conductivity and Development Intensity

32. Contaminants: Preliminary Results Lithium Carbamazepine (anticonvulsant/mood disorders) Sulfamethoxazole (antibiotic) Gadolinium (Sewer Adjacent Ponds) Atrazine not detected

33. Estrogens in Forested, Sewer, and Septic Ponds

34. Landscape Influences

36. Sex Ratio and Development Intensity

38. What do we do? Household Scale Septic Filter?

39. Conclusions - Intersex Frogs Common, Sex Ratio Variable - Land Cover Strongly Predictive (Suburbs) - Contamination Diverse, Pervasive; Implies Domestic Origin - Implications for Sex Determination