1. THE OBTAINING OF A NEW NATURAL VEGETAL PRODUCT HOF.LIPOMIN HAVING HYPOLIPEMIANT PROPERTIES GABRIELA DENISA RIZEA 1 *, DENISA MIHELE 2, DANIELA IONESCU 1, ŞTEFAN MANEA 1, MARIA IVOPOL 1 OBJECTIVES The obtaining of the new natural vegetal hypolipemiant product named Hof.Lipomin having in composition the inulinic concentrated and degreased extract of Helianthi tuberosus tuber, the Linseed oil, the extracts of Allii sativi bulbus and Allium porrum.The determination of the chemical composition of the obtained product, of its physical chemical and microbiological properties as well as the emphasing of its hypolipemiant action on the laboratory animals and in clinical studies. MATERIALS AND METHODS The chemical compostion was established using HPLC, UV-VIS, and AEA Spectrometry methods. The hypolipemiant activity was determined on the white masculs Wistar rats, having 220-250g. The clinical studies were realized on 30 patients having dislipemia monitorized over a period of 120 days. RESULTS Biochemical parameters of the animals treated with Hof.Lipomin, having WR 1339 Triton induced hyperlipemia Figure 1. The effect of Hof.Lipomin and its constituients Figure 2. The effect of Hof.Lipomin on LDL- cholesterol of the rats on total serum lipids of the rats CLINICAL STUDIES Nr. crt. Patients The before cholesterol total [mg/dL] The total cholesterol after [mg/dL] Triglycerides before [mg/dL] Triglyceride s after [mg/dL] 1V.A.240220 100201 2S.F.260208 8060 3R.L.180167 101102 4S.A.218220 10260 5A.V.157180 60210 6S.P.236202 180190 7P.I.300240 430180 8G.M.282262 11080 9A.V.180182 14090 10D.G.260180 130132 11S.C.180170 150160 12I.V.310230 350170 13P.M.220180 170240 14S.I.260250 330430 Average234,5 ± 20,15 206,5 ± 15,5 173,48± 20,15178,81± 15,15 Figure 3. Graphic representation the obtained results for the total cholesterol Figure 4. Graphic representation the obtained results for the serum triglycerides CONCLUSIONS: The new product is in accordingly to the FR X concerning the phisico-chemical and microbiological properties. Hof.Lipomin doesn’t present cytotoxicity on the cells cultures. The studies realized on the animals experiments emphasized the lowering of the total serum lipids, of the total serum cholesterol and of serum triglycerides. The clinical studies concerning the application of the Hof.Lipomin in treatment of dislipemies point aut the decrease of total serum lipids, serum cholesterol, LDL cholesterol and serum triglycerides. The ratio total cholesterol /HDL cholesterol was improved by the total serum cholesterol lowering and by the HDL increasing. The obtained results prove that it is possible to decrease the risk of dangerous cardio-vascular events using Hof.Lipomin. REFERENCES 1. Arnault, J, Auger J., Selenocompousse in garlic and onion, Journal of Chromatography, 2006,112 (1-2), 23-30. 2. Mihele D., Biochimie chimica, Metode de laborator, ed. III, Ed. Medicala, 2007, Bucuresti 3.Rizea G.D., Popescu M., Ionescu D., Mihele D., Manea Ş., 2012,,Comparative Determinations of Antioxidant Free Radical Scavenging Polyphenols, in Certain Natural Products by HPLC Methods and UV-Vis Spectrophometry’’, Rev.Chim.(Bucharest),63(11), 1085-1088.