1. Biosensors And how they are changing the world

2. What are they? A device that utilizes biological components to indicate the amount of a biomaterial

3. Breakdown of Components. Bioreceptor- detects analyte Transducer- converts into readable form Reader Device- displays information

6. Biosensors in the Future What should a biosensor be? Portable, easy to use, small Easy to be manufactured in mass quantities Low cost, affordable Rapid results Satisfy a large market

7. Military Use The military has funded research into biosensors Mine sensing bacteria Monitoring gasses on the battlefield Detecting air-borne pathogens To counter Bio-terrorism

8. Agriculture Detecting heavy metal ions, arsenic, ammonia in water sources Brazilian researchers developing a pesticide detector

9. Medical Detecting bladder cancer and lyme disease “Artificial Skin” using flexible biosensors that can aid in prosthetics Glucose and Pregnancy Tests Cholesterol Test was a failure

10. Design Project Factor VIII producing E. coli

11. What is Hemophilia A Hemophilia affects the ability of the body to control blood clotting or coagulation Hemophilia A is a genetic blood disorder that is caused by a Factor VIII deficiency Hemophilia affects more than 17,000 people in the United States

13. Current Treatment Options Replacement therapy Desmopressin Antifibrinolytic Medicine

14. Proposal Using E. coli, create a topical creme containing bacteria engineered to produce clotting factor VIII in order to help close external wounds for people with Hemophilia A

15. LuxS ABC Transporter Al-2 ATP ADP LsrR P P Factor VIII ATP ADP Factor VIII molecules Export ATP ADP LuxS Porcine Coagulation Factor VIII LSR Transport Cassette Fibrin

16. Advantages Low Risk, using Pig Factor VIII No chance of blood-transmitted disease Works even with antibodies and inhibitors Site Specific No Injection required

17. Improvements Kill Switch Does not solve internal bleeding Separate design needed for Hemophilia B