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Oral Spot Practical Examination (OSPE) - 2

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Presentation on theme: "Oral Spot Practical Examination (OSPE) - 2"— Presentation transcript:

1 Oral Spot Practical Examination (OSPE) - 2

2

3 Shigella on Deoxycholate citrate agar (DCA)

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5 Salmonella on Salmonella-Shigella (SS) agar.

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8 API-20 strip test

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10 Proteus: Urease positive

11 Differences between Klebsiella, Enterobacter, Serratia
Character Klebsiella Enterobacter Serratia Motility - + Indole Red pigment Quellung reaction

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13 Widal tube agglutination test

14 Interpretation of Widal test
Condition Doubtful Significant Typhoid Fever 1:160 1:320 Paratyphoid Fever 1:80

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16 Colicine typing of Shigella

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18 Swarming of Proteus

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20 Pseudomonas on Blood agar

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22 CETRIMIDE AGAR: Selective for P. aeruginosa

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24 Positive Citrate test for Pseudomonas

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26 Pseudomonas pyocine typing

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28 Bacteroides fragilis: Neomycin selective medium

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30 Bacteroides sensitive to metronidazole disc

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32 Campylobacter Gram negative smear

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34 Campylobacter on Butzler medium

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36 C. jejuni hydrolyze hippurate

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38 Gram stain of Vibrio cholerae

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40 TCBS Agar - Vibrio

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42 Alkaline peptone water and Selenite - F

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44 V. Cholerae : Cholera red reaction

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46 Haemophilus culture on chocolate agar

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48 Haemophilus influenzae: X and V factors

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50 Carbon dioxide candle jar

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52 Fluorescent antibody stain.

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54 Pernasal swabs

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56 Bordetella on Bordet - Gengou medium

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58 Bordetella on selective charcoal cephalexin blood agar

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60 Slide latex agglutination test with specific antisera

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62 Yersinia bipolar safety pin Giemsa staining

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64 Yersinia: non-lactose fermenter on Mc Conkey

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66 Francisella tularensis: on chocolate cysteine agar

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68 Pasteurella multocida: non-hemolytic, mucoid colonies

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70 Blood culture bottles

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72 Brucella: Blood culture Castaneda diphasic bottle

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74 Brucella on tryptone soya agar

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76 B. abortus & meletensis grow in presence of basic fuchsin

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78 Brucella : Standard Agglutination Test

79 Identification of Brucella species
CO2 H2S (days) Growth in presence of Urease 1 2 3 4 5 Basic fuchsin Thionin Methyl violet B. melitensis - + + / - B. abortus B. suis

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81 Blood wet preparation for Borrelia

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83 Leptospira interrogans

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85 Darkfield microscope

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87 Treponema pallidum

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89 Treponema pertenue

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91 Treponema carateum

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93 VDRL TEST

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95 Treponema Pallidum Immobilization

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97 Treponema pallidum Hemagglutination test

98 Comparison of VDRL AND RPR
Specimen Only serum used, serum heated Serum & plasma used, no heating Antigen Diluted before use Not diluted Reading of results Microscopically from slide Macroscopically from card Technique Difficult Easy Sensitivity Less sensitive More sensitive False positive Less often More often

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100 Legionella pneumophila: Gram stain

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102 L. pneumophila on selective Mueller Hinton agar

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104 Immunofluorescence Microscope

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106 Branching rods of Nocardia asteroides

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108 Gram-positive branching rods of Actinomyces

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110 Sulpher granules of Actinomyces

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112 Disc diffusion sensitivity test

113 Control Organism Test Organism

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116 E - TEST SENSITIVITY TECHNIQUE

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118 Rosco Neo-Sensitabs Sensitivity Test

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120 Antibiotics synergy test on Mueller–Hinton agar

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122 Antibiotics antagonism on Mueller–Hinton agar

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124 Chlamydia - Giemsa stain

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126 Chlamydia Mc Coy tissue culture

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128 Chlamydia - CFT

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130 Mycoplasma agar: Colonies have fried egg shape

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132 Mycoplasma : A positive cold agglutinin test

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134 Sites of inoculation of fertile eggs
Coxiella: Culture in yolk sac of chick embryo

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136 Giemsa stain: Rickettsia inside the cell

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138 Rickettsia: Weil-Felix Test

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140 Helicobacter pylori: Spiral gram negative rods

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142 Helicobacter pylori on selective Butzler medium

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144 Water Examination: The multiple tube technique

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146 Water Examination: Viable plate count

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148 Milk Examination: Methlylene blue reduction test

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150 Milk Examination: Milk ring test:

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152 Milk Examination: Phosphatase test:

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