1. Supplemental Data Fig. 1A. Standard curves of miR-34c-5p using synthetic miRNAs.

2. Supplemental Data Fig. 1B. Standard curves of miR-122 using synthetic miRNAs.

3. Supplemental Data Fig. 1C. Standard curves of miR-146b-5p using synthetic miRNAs.

4. Supplemental Data Fig. 1D. Standard curves of miR-181a using synthetic miRNAs.

5. Supplemental Data Fig. 1E. Standard curves of miR-374b using synthetic miRNAs.

6. Supplemental Data Fig. 1F. Standard curves of miR-509-5p using synthetic miRNAs.

7. Supplemental Data Fig. 1G. Standard curves of miR-513a-5p using synthetic miRNAs.

8. Supplemental Data Fig. 2A. The repeatability of RNA extraction for the analytical characterization of hydrolysis probe-based RT-qPCR assay.

9. Supplemental Data Fig. 2B. The repeatability of RT-qPCR assay for the analytical characterization of hydrolysis probe-based RT-qPCR assay.

10. Supplemental Data Fig. 3A. The concentration of semen sperm miR-34c-5p in 15 infertile patients with asthenospermia and 12 fertile controls.

11. Supplemental Data Fig. 3B. The concentration of semen sperm miR-122 in 15 infertile patients with asthenospermia and 12 fertile controls.

12. Supplemental Data Fig. 3C. The concentration of semen sperm miR-146b-5p in 15 infertile patients with asthenospermia and 12 fertile controls.

13. Supplemental Data Fig. 3C. The concentration of semen sperm miR-181a in 15 infertile patients with asthenospermia and 12 fertile controls.