1. Emiliania huxleyi increases calcification but not expression of calcification-related genes in long-term exposure to elevated temperature and pCO 2 by Ina Benner, Rachel E. Diner, Stephane C. Lefebvre, Dian Li, Tomoko Komada, Edward J. Carpenter, and Jonathon H. Stillman Philosophical Transactions B Volume 368(1627):20130049 October 5, 2013 ©2013 by The Royal Society

2. Carbon to nitrogen (C : N) ratio of the E. huxleyi ((Lohm.) Hay and Mohler) cells at 215, 414 and 703 generation points under present ocean condition (383 ± 43 µatm pCO2, 20.0 ± 0.1°C; white bars) and future ocean condition (833 ± 68 µatm pCO2, 24.0 ± 0.2°C... Ina Benner et al. Phil. Trans. R. Soc. B 2013;368:20130049 ©2013 by The Royal Society

3. (a) PIC and (b) POC content of Emiliania huxleyi at 215, 414 and 703 generation points under present ocean condition (383 ± 43 µatm pCO2, 20.0 ± 0.1°C; white bars) and future ocean condition (833 ± 68 µatm pCO2, 24.0 ± 0.2°C; black bars). Ina Benner et al. Phil. Trans. R. Soc. B 2013;368:20130049 ©2013 by The Royal Society

4. (a) Calcification rate and primary production (b) of Emiliania huxleyi at 215 and 703 generation points under present ocean condition (383 ± 43 µatm pCO2, 20.0 ± 0.1°C; white bars) and future ocean condition (833 ± 68 µatm pCO2, 24.0 ± 0.2°C; black bars). Ina Benner et al. Phil. Trans. R. Soc. B 2013;368:20130049 ©2013 by The Royal Society

5. Downregulated putative transcripts in Emiliania huxleyi grown under future ocean conditions at the 215 generation point. Ina Benner et al. Phil. Trans. R. Soc. B 2013;368:20130049 ©2013 by The Royal Society

6. Functional categorization of the upregulated putative transcripts in Emiliania huxleyi grown under future ocean conditions at the 215 generation point. Ina Benner et al. Phil. Trans. R. Soc. B 2013;368:20130049 ©2013 by The Royal Society

7. Major pathways in each functional categorization from figure 5 that were upregulated in future ocean conditions at the 215 generation point. Ina Benner et al. Phil. Trans. R. Soc. B 2013;368:20130049 ©2013 by The Royal Society

8. Quantitative real-time PCR (qPCR) data for calcification-related genes selected from the literature. Ina Benner et al. Phil. Trans. R. Soc. B 2013;368:20130049 ©2013 by The Royal Society