1. Herpes viruses. Adenoviruses. Biological properties, pathogenecity for humans. Laboratory diagnostics, prophylaxis and therapy of diseases. Vinnitsa National Pirogov Memorial Medical University / Department of microbiology

2. Herpes viruses. Classification 1. Alphaherpesvirinae includes next species 1. Herpes simplex viruses 1 and 2 (HSV1 and HSV2) 2. Varicella-zoster virus (VZV or HHV 3) 2. Betaherpesvirinae contains species 1. Cytomegalovirus (CMV or HHV 5) 2. HHV6 and HHV7 3. Gammaherpesvirinae includes species 1. Epstein-Barr virus (EBV or HHV4) 2. Kaposi`s sarcoma associated virus (HHV8)

3. Herpes viruses

4. Herpes simplex virus There are two types of the herpes simplex virus (HSV): 1. HSV type 1 causes herpes labialis 2. HSV type 2 causes herpes genitalis

5. Cultivation of the HSV 1. Chick embryo 2. Cell cultures 3. Experimental keratoconjunctivitis

6. Epidemiology and pathogenesis The source of infection is ill person with typical lesions Infection is transmitted by close direct contact (labial herpes) or sexual intercourse (genital herpes)

7. Laboratory diagnostics Microscopy: Cytoscopy Immune electron microscopy Immunofluorescence Virus isolation Serology

8. Varicella-zoster virus (VZV) causes chickenpox after primary infection and herpes zoster The source of infection is person with chickenpox or more rarely with herpes zoster Infection is transmitted with air droplets (chickenpox) or with direct contact with lesions

9. Varicella (chickenpox) Herpes zoster

10. Pathogenesis of varicella/zoster infection

11. Laboratory diagnostics Microscopy: Tzanck smear Immune electron microscopy Immunofluorescence Virus isolation Serology

12. Prevention is possible by active immunization with live, attenuated VZV (OKA strain) For contact person varicella-zoster immunoglobulin (VZIG) is used to prevent disease

13. Cytomegalovirus (CMV) In persons with adequate immunity CMV causes subclinical or unapparent infection. In persons with waned immunity CMV can provoke generalized infection. Congenital CMV-infection often is very severe, associated with hepatosplenomegaly, jaundice, hemolytic anemia, and microcephaly, chorioretinitis

14. Cells infected by CMV

15. Laboratory diagnostics Rapid diagnostics Virus isolation Serological method

16. Epstein-Barr virus (EBV) It belongs to gamma-herpes viruses (HHV-4) and has oncogenic properties. It can cause different diseases from latent infection in children, infectious mononucleosis in adulthood to EBV- associated malignancies such as Burkitt`s lymphoma and nasopharingeal carcinoma

17. Laboratory diagnostics 1. Blood assay 2. Serological tests are used to reveal: Heterophile antibodies with Paul-Bunnell test Specific anti-EBV antibodies with ELISA and immunofluorescence

18. Adenoviruses They belong to family Adenoviridae, genus Mastadenovirus divided into 7 serogroups (A-F) and 47 serotypes

19. Adenovirus structure

20. Pathogenecity 1. Respiratory disease in children (pharyngitis, pneumonia) 2. “Common cold” in adults 3. Conjunctivitis (swimming pool follicular conjunctivitis and keratoconjunctivitis -shipyard disease) 4. Diarrhea in children 5. Acute hemorrhagic cystitis in young males

21. Epidemiology and pathogenesis Adenoviruses are transmitted with different mechanisms: 1. Via aerosol droplets 2. With fecal-oral route 3. By direct contact

22. Laboratory diagnostics Diagnosis is based onto: 1. Rapid diagnostics with electron microscopy, immune electron microscopy, immunofluorescence 2. Virus isolation 3. Serological tests demonstrate rising in titer of antibody, demonstrated in paired patient sera