1. Preliminary Detailed Design Review P15080 Flow Culture System November 13, 2014

2. Engineering Requirements ● No changes have been made since last meeting ● Document is under change control ● Most important ERs are: o Shear Stress o Assembly Time o Size of Flow Channel EDGE Link

3. System Model/Architecture/Design Growth chamber design changes: o Minor updates to system architecture diagram and interfaces document o Generated new 3-D model of growth chamber o Verified chamber can be integrated into system o Verifying lid thickness and attachment mechanism is appropriate Chamber/System Integration Link

4. Pump Cell Chamber Media Reservoir Electricity Flow Rate Cells Microscopes Alternate Reservoir Media Media Color HeatCO 2 Water Pan ArchitectureColor Structure Energy Information Materials Incubator Filter System Architecture Media in Tubing Light CO 2 (moves while still attached) Pump Setting (swap) (pumped into incubator) Heating Element Power Cord Fresh Media (seeded) Microscope Slide Lid (in a groove with a gasket) Base 4 Bolts and 4 Wing Nuts (lid has gasket)

5. Growth Chamber Wing Nuts Lid Cap Metric Microscope Slides Lid and Slide Gaskets Body Base Cap Bolts ●Aluminum 6061 10” x 2.5” x 0.5” body sandwiched by two layers of 0.25” polycarbonate ●1 mm x 1 mm rubber gasket fits around channel, bolts keep lid pressed down to seal top of channel ●1 mm x 2 mm rubber gasket fits around either slide recession, tight fit of slides maintains a seal on bottom of channel ●Four 0.25” bolts run through corners of chamber and keep the system together, used with wing nuts to be tightened manually

6. Growth Chamber Connections Threaded Inlet/Outlet Holes Threaded Luer Adapter Male Luer Adapter 3-Way Stopcock Female Luer Adapter ●All components are polycarbonate and are autoclavable ●Threaded Luer adapters fit into the chamber inlet and outlet holes ●The threaded Luer adapters connect to a male or female Luer adapter each (both are compatible) ●A short section of tubing will be attached next ●A female or male Luer adapter connects to both open tubing ends ●3-way stopcocks connect to the Luer adapters, oriented as needed (to accommodate a male or female adapter) ●A second 3-way stopcock will be added to each side ●The appropriate Luer adapter (opposite of the last type used) connects to the open stopcocks ●The system’s inlet and outlet tubes can now connect to these adapters and connect the growth chamber

7. Feasibility Analysis: Lid Deflection

8. Feasibility Analysis: Flow Required to achieve 2 Pa of shear stress on cells. Q = 640 mL/min

9. Feasibility Analysis: Flow Pressure Drop is approximately 582 Pa

10. Feasibility Analysis: Flow Conclusion: Design is feasible! ●Reaches 2 Pa requirement Pump requirements confirmed. ●Flow range ●Tubing Size

11. CAD Drawing

15. Risk Analysis ●Risk is decreasing nicely ●Largest Risks ○ Control of pump ○ Sterility ○ Incubator/Pump Interface ●All risks that had a deadline of 11/13/14 to be driven to zero are zero ●Linked parts on BOM to Risk Analysis ●Linked risks to feasibility models ○ Some feasibility still in progress ●Linked risks to Test Plans

16. Project Plan

17. To Do: ● Deflection Discussion with SME o Develop COMSOL deflection model and verify Analytical Model ● Final Drawings o Draft Final Drawings o Brinkman evaluation of tolerances/design and quote ● Quotes and Purchase Orders and Lead Times o Tubing, Luers, Stopcocks ● Begin Pump Testing o Locate Tubing within BME Dept.

18. Critical Path Into Phase 5

19. Efficiency ● Average Efficiency Last Phase:70% ● Average Efficiency This Phase: 89% Overall efficiency has improved this phase o Realistic Deadlines o Better communication ● Ways to Improve Further o Consider other coursework when setting deadlines

20. HW/SW Design This is not applicable to our project.

21. Initial Budget and BOM ● Ordered Pump ● Total Projected Budget is $957.65 o Need to request quotes o Need to finalize shipping costs and discounts ● Parts linked to risks ● Part numbers linked to design drawings Link

22. Part-Linked Media System

23. Part-Linked Growth Chamber

24. Part-Linked Complete System Not Shown: ● Incubator ● Cells ● Gaskets ● Nuts ● Bolts

25. Assembly Process ● Previously completed a preliminary assembly procedure ● Revisions to account for growth chamber design changes ● Likely will be adjusted during construction in MSD II

26. Test Plan Outline ● Added two test plans o Tubing in Incubator Door o Blockage of Flow-Ajar Microscopes ● Plan to add more as ideas come and materials arrive Link

27. Self Assessment ● Important to have accountability partners o Have others double check work! ● Important to ask for other group members opinions ● Recognize costs - they add up quickly! ● Balance with other coursework