1. MECHANIZATION OF LILY MICROBULB MULTIPLICATION OPERATIONS Ta-Te Lin and Ching-Lu Hsieh Department of Agricultural Machinery Engineering, National Taiwan University, Taipei, Taiwan, ROC

2. MECHANIZATION OF LILY MICROBULB MULTIPLICATION OPERATIONS Ta-Te Lin and Ching-Lu Hsieh Department of Agricultural Machinery Engineering, National Taiwan University

3. n INTRODUCTION n MODELING n PROCESS OPTIMIZATION n MECHANIZATION OF MULTIPLICATION PROCESS n CONCLUSIONS

4. INTRODUCTION n Lily microbulb tissue culture cycle n Microbulb dissecting and transplanting

5. LILY MICROBULB TISSUE CULTURE CYCLE

7. LILY MICROBULB IN CULTURE VESSEL

8. MICROBULB DISSECTING AND TRANSPLANTING n Vessel opening n Bulb gripping n Root and leaf removal n Bulb grading n Bulb scale separation n Scale transplanting n Vessel sealing n Vessel labeling

9. MODELING n Analysis of manual operation n Batch process model n Stepwise process model

10. ANALYSIS OF MANUAL OPERATION

13. FLOW CHART OF LILY MICROBULB MULTIPLICATION PROCESS

14. BATCH PROCESS MODEL

15. STEPWISE PROCESS MODEL

16. Probability of measured entry vessel quantity with fitted lognormal density function

17. Probability of measured single vessel processing time with fitted lognormal density function

18. Probability of measured propagation rate with fitted lognormal density function

19. Total processing time, as affected by entry vessel quantity under various single vessel processing times (ST)

20. Finished vessel quantity, as affected by entry vessel quantity under various multiplication rates (MR)

21. Contour plot of predicted finished vessel quantities (dotted lines) and total processing times (solid lines)

23. PROCESS OPTIMIZATION n Response surface method (RSM) n Optimum analysis

24. RESPONSE SURFACE METHOD n Experimental design n Parameter estimation n Reliability test n Response surface examination

25. EXPERIMENTAL DESIGN n Dependent variables Separation rateSeparation rate Injury rateInjury rate n Independent variables Cutting positionCutting position Spinning speedSpinning speed Separation timeSeparation time

26. Values of the coded and uncoded independent variables in the RSM analysis of microbulb scale separation operation

27. Experimental conditions of the Box-Behnken experimental design for RSM analysis and the experimental results

28. Coefficients of the regressed 2nd order polynomial equations for separation rate and injury rate

29. Predicted separation rate (solid line) and injury rate (dotted line) for microbulb of cutting position A

30. Predicted separation rate (solid line) and injury rate (dotted line) for microbulb of cutting position B

31. Predicted separation rate (solid line) and injury rate (dotted line) for microbulb of cutting position D

32. Comparison between predicted and measured separation rate, injury rate and propagation rate of the validation experiment

33. MECHANIZATION OF MULTIPLICATION PROCESS n Scale separation n Scale transplanting n Other mechanical components

34. SCALE SEPARATION

35. Separation rate of lily microbulb with cutting position A as affected by separation time, spinning speed

36. Separation rate of lily microbulb with cutting position B as affected by separation time, spinning speed

37. Separation rate of lily microbulb with cutting position C as affected by separation time, spinning speed

38. Separation rate of lily microbulb with cutting position D as affected by separation time, spinning speed

39. Injury rate of lily microbulb with cutting position A as affected by separation time, spinning speed

40. Injury rate of lily microbulb with cutting position B as affected by separation time, spinning speed

41. Injury rate of lily microbulb with cutting position C as affected by separation time, spinning speed

42. Injury rate of lily microbulb with cutting position D as affected by separation time, spinning speed

43. SCALE TRANSPLANTING

44. CONCLUSIONS n n The bulb scale separation and transplanting operation was identified as the most laborious operation in the process. n n A batch-type model and a stepwise model were constructed to study the influence of operation parameters. n n At an optimum spinning speed and separation time, lily microbulb could be successfully separated into scales with acceptable injury rate. n n A bulb scale separation and transplanting machine was developed and the process was optimized.

45. THANK YOU 謝 謝