2. THE MOST IMPORTANT BIOLOGY LESSON OF THE YEAR

3. How does DNA work?

4. The “Central Dogma” DNA

5. The “Central Dogma” DNA RNA

6. The “Central Dogma” DNA RNA proteins

7. The “Central Dogma” DNA RNA proteins you

8. RNA Five differences between RNA and DNA 1.Sugar in RNA is ribose instead of deoxyribose 2.RNA is single stranded 3.RNA contains Uracil instead of Thymine 4.RNA is disposable 5.RNA can be outside the nucleus, DNA can’t If you are going from DNA to RNA – what nitrogenous base would pair with Adenine? DNA: C A G T T A RNA: _ _ _ _ _ _

9. ANSWER: DNA: C A G T T A RNA: G U C A A U

10. Types of RNA Key players in Protein Synthesis - –Messenger RNA (mRNA): carry instruction copies –Ribosomal RNA (rRNA): makes up ribosome along with proteins –Transfer RNA (tRNA): brings amino acids to the ribosome

11. Big picture of protein synthesis

12. Transcription

13. Translation

14. Just Like Construction … DNA is the master plan mRNA is the everyday blueprint rRNA is the builder tRNA is the gopher Amino Acids are the wood Proteins are the building

15. Translation of the Genetic Code - Protein Synthesis

16. A U G G G C U U A A A G C A G U G C A C G U U This is a molecule of messenger RNA. mRNA is transcribed in the nucleus. mRNA molecule codon

17. A U G G G C U U A A A G C A G U G C A C G U U A ribosome attaches to the mRNA molecule. ribosome

18. A U G G G C U U A A A G C A G U G C A C G U U It brings a specific amino acid to the first three bases (codon) on the mRNA. Amino acid tRNA molecule anticodon U A C A transfer RNA molecule arrives. The three unpaired bases (anticodon) on the tRNA link up with the codon.

19. A U G G G C U U A A A G C A G U G C A C G U U Another tRNA molecule comes into place, bringing a second amino acid. U A C C C G Its anticodon links up with the second codon on the mRNA.

20. A U G G G C U U A A A G C A G U G C A C G U U A peptide bond forms between the two amino acids. Peptide bond C C G U A C

21. A U G G G C U U A A A G C A G U G C A C G U U The first tRNA molecule releases its amino acid and moves off into the cytoplasm. C C G U A C

22. A U G G G C U U A A A G C A G U G C A C G U U C C G The ribosome moves along the mRNA to the next codon.

23. A U G G G C U U A A A G C A G U G C A C G U U Another tRNA molecule brings the next amino acid into place. C C G A A U

24. A U G G G C U U A A A G C A G U G C A C G U U A peptide bond joins the second and third amino acids to form a polypeptide chain. C C G

25. A U G G G C U U A A A G C A G U G C A C G U U The polypeptide chain gets longer. G U C A C G The process continues. This continues until a termination (stop) codon is reached. The polypeptide is then complete.

26. CODON TABLE

27. 1. What amino acids would be coded by the following DNA sequence? CGGACCGCTATC 2. What would be the mRNA sequence for a polypeptide chain (protein) that had the following amino acids? DNA sequence? Lysine-Cysteine-Proline