1. rh Lecture 6 Slides

2. Go to TA sections; challenge them! Come to R’s and/or TAs office hours 2130 Pac Hall Mon 2-3 Tue 2-3 details on course web site: http://course.ucsd.edu/rhampton/bibc102

3. Warburg Effect the Warburg effect is the observation that most cancer cells predominantly produce energy by a high rate of glycolysis followed by lactic acid fermentation in the cytosol Otto Heinrich Warburg 1931 Nobel Prize in Medicine

4. Warburg Effect

5. fig 14-10 Getting other sugars into the glycolytic pathway

6. pg 545 A second route for fructose (in liver)

7. Glycogen: nature’s sugar bowl

8. fig 14-11 more about glycogen later Liberating glucose units from glycogen

9. From glycogen to glycolysis… phosphogluco- mutase another mutase…

10. fig 14-1 Now what?

12. fig 14-1 Now what?

13. fig 14-1 Now what? Fermentation: anaerobic metabolism of glucose without oxidation...

14. pg 547 LDH converts pyruvate to lactate, restoring the pool of NAD +

15. pg 547 LDH converts pyruvate to lactate, restoring the pool of NAD +

16. pg 547 ethanol production: another NAD + restoration strategy

17. fig 14-14 Thiamine pyrophosphate (TPP): an activated carbon

18. fig 14-14 Thiamine pyrophosphate in ethanol production

19. fig 14-14 TPP as a carbanion nucleophile in pyruvate metabolism

20. fig 14-14 TPP as a carbanion nucleophile in pyruvate metabolism

21. fig 14-14 TPP as a carbanion nucleophile in pyruvate metabolism

22. TPP-mediated enzymatic reactions TPP is all about carbonyl activation table 14-1

23. The glycolysis energy landscape (pyruvate set to 0)

24. Regulated glycolytic enzymes hexokinse (and glucokinse) phosphofructokinase (PFK-1) pyruvate kinase regulated by G6P allosterically liver isozyme is glucokinase, different regulated by ATP, citrate, and fatty acids

25. The glycolysis energy landscape (pyruvate set to 0)

26. regulation of hexokinase: isozymes can differ fig 15-12

27. fig 15-14 PFK-1 : a right fancy enzyme!

28. fig 15-14 PFK-1: allosteric regulation by ATP, etc…

29. PFK-1: allosteric regulation by ADP, etc… xxx

30. fig 15-14 FPK-1 has multiple allosteric regulators

31. fig 15-14 Our book’s notation for regulators inhibitionactivation

32. fig 15-14 PFK-1 has multiple allosteric regulators

33. fig 14-21 Glucose as a source of other stuff Pentose phosphate pathway oxidative reactions

34. fig 14-21 Pentose phosphate pathway oxidative reactions

35. fig 14-21 Pentose phosphate pathway oxidative reactions

36. fig 14-21 Pentose phosphate pathway oxidative reactions

37. fig 14-22 Pentose phosphate pathway: non-oxidative rxns all movement of carbonyl groups to and fro…

38. fig 14-22 Pentose phosphate pathway: non- oxidative reactions 2C 3C2C 3C end of lecture 6

39. pg 547 LDH converts pyruvate to lactate, restoring the pool of NAD +