1. DNA Chapter 12-1

2. Role of DNA Genetic basis of life Carries code for all the genes of an organism Genes create proteins Proteins perform life functions Template for future generations

3. DNA is a DOUBLE HELIX http://en.wikipedia.org/wiki/Rosalind_Franklin http://www.time.com/time/time100/scientist/profile/watsoncrick.html X-ray experiments by Rosalind Franklin led James Watson and Francis Crick to the discovery of the structure of DNA in 1953

4. NITROGEN BASES in DNA _____________= A _____________ = G _____________ = C ______________ = T ADENINE GUANINE CYTOSINE THYMINE

5. DEOXYRIBONUCLEIC ACID Image from: http://www.tokyo-med.ac.jp/genet/picts/dna.jpg ______________ STRANDED Backbone (sides of ladder) made of _____________ and _____________ DOUBLE PHOSPHATES sugars

6. Purines (2 rings) Pyrimidines (1 ring) A G C T Phosphate group Deoxyribose sugar Nitrogen bases =“Steps of ladder” © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved

7. DOUBLE HELIX Hydrogen Image from: http://evolution.berkeley.edu/evosite/evo101/images/dna_bases.gif _____________ bonds between nitrogen bases hold the two strands together.

8. CHARGAFF’S RULES A = T G = C Image from: http://evolution.berkeley.edu/evosite/evo101/images/dna_bases.gif _________ At time no one knew why… now we know its because Adenine always bonds across with____________ Guanine always bonds across with ____________ THYMINE CYTOSINE

9. CHROMOSOMES & DNA REPLICATION 12-2

10. Chromosome E. coli bacterium Bases on the chromosome Chromosome Structure in Prokaryotes © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved DNA molecule in bacteria is: ______________ Found in __________ (NO nucleus) SINGLE CIRCULAR CYTOPLASM Approximately 5 million base pairs 3,000 genes

11. DNA in EUKARYOTES is packaged into chromosomes Humans have approximately 3 billion base pairs (1 m long) 60,000 to 100,000 genes If the diameter of the DNA (2 nanometers) was as wide as a fishing line (0.5 millimeters) it might stretch as far as 21.2 km (or 13.6 miles) in length which would all have to be packed into a nucleus, the equivalent size of 25 cm in diameter. That is some packaging! http://www.paternityexperts.com/images/DNA-of-life.jpg

12. THINK ABOUT IT How could you get this piece of string into the container? http://www.artzooks.com/files/3966/AZ533823_320.jpg http://www.mivaroo.com/sites/toyconnection.com/

13. Chromosome Structure of Eukaryotes Chromosome Supercoils Coils Nucleosome Histones DNA double helix © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved

14. Replication is the process where DNA makes a copy of itself.

15. WHY????? Simple: Cells divide for an organism to grow or reproduce, every new cell needs a copy of the DNA or instructions to know how to be a cell. DNA replicates right before a cell divides.

16. DNA replication is semi-conservative. That means that when it makes a copy, one half of the old strand is always kept in the new strand. This helps reduce the number of copy errors

17. So why do we have all this stuff

18. Protein Synthesis Assembly of proteins – Occurs on ribosome Two Primary Steps – Transcription-RNA is made from DNA – Translation- Proteins are made from the message on the RNA

19. Why Proteins Enzymes – help carry out reactions within the cell Hormones – chemical messengers that regulate body functions Provide structure Energy source Transport other molecules Part of our immune system

20. RNA

21. RNA- the Other Nucleic Acid Also made of ___________ Sugar is _______ instead of deoxyribose. RNA is _________ stranded Contains _________ instead of thymine. NUCLEOTIDES RIBOSE SINGLE URACIL http://images2.clinicaltools.com/images/gene/dna_versus_rna_reversed.jpg

22. 3 KINDS OF RNA HELP WITH INFO TRANSFER FOR PROTEIN SYNTHESIS _________________RNA (rRNA) Combines with proteins to form ribosomes _________________RNA (tRNA) Matches m-RNA codon to add correct amino acids during protein synthesis _________________RNA (mRNA) carries code from DNA to ribosomes rRNA and t-RNA images from © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved mRNA image from http://wps.prenhall.com/wps/media/tmp/labeling/1140654_dyn.gif RIBOSOMAL TRANSFER MESSENGER

23. RNA DNA RNA polymerase Figure 12–14 Transcription Section 12-3 Adenine (DNA and RNA) Cystosine (DNA and RNA) Guanine(DNA and RNA) Thymine (DNA only) Uracil (RNA only) Enzyme called _____________________ separates strands, then uses one strand as a template to assemble an RNA copy. RNA POLYMERASE

24. MASTER PLAN DNA stays safe in nucleus TRANSCRIPTION (DNA → RNA) & PROCESSING takes place in nucleus TRANSLATION (RNA → proteins) takes place on ribosomes in cytoplasm © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved “Blueprints” of master plan are carried to building site http://www.home-improvement-resource.com/images/architect.jpg

25. HOW CAN JUST 4 BASES GIVE DIRECTIONS TO MAKE 20 AMINO ACIDS? Message is read in groups of 3 = _________ UCGCACGGU UCG-CAC-GGU CODON Codons represent different amino acids Serine- Histidine- Glycine

26. The m-RNA Code Section 12-3 64 possible codons Some amino acids have more than one codon. START= _______ 3 codons for _____ AUG STOP

27. ___________ on tRNA matches up with ________ on mRNA Images modified from © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved ANTICODON CODON EACH tRNA carries only one kind of _____________ amino acid

28. GENES & PROTEINS Proteins are the connection between the gene code in the DNA and how that gene is expressed. A gene that codes for an enzyme (protein) to make a pigment can control the color of a flower. A gene that codes for an enzyme (protein) adds carbohydrates to glycoproteins to produce your blood type. Enzymes catalyze and regulate chemical reactions so proteins build and operate all cell components. Mendel/flower images from: http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookTOC.html Blood cell by Riedell

29. DNA → DNA ____________ DNA → RNA ____________ RNA → Protein ___________ REPLICATION TRANSCRIPTION TRANSLATION