1. 李逊斗，高友鹤 教授 中国医学科学院基础医学研究所 药物对尿蛋白质组的影响

2. Biomarker

3. Factors affect the urine Urine Environmental factors Medication Etc. Oral diet Stress Age Gender 骨折 Smoking Exercises

4. Biomarker discovery Healthy controls Patients Medications No or rare medications

5. Pharmuromics Pharm-ur-omics  Uromics: urinary proteomics, metabolomics, DNAs, miRNAs, etc.  Pharmuromics: effects of commonly used medications on the uromics.  Pharmuromic Database  Disease specific biomarkers

6. Strategy  Single factor study  Human  Model animals  Genetic background  Diet  Living Environment  etc.

7. Diuretics Affect urine volume and salts Urinary proteins? Kidney disease nephritis, renal failure HypertensionHeart failureEdemaCirrhosis

8. Diuretics Thiazide diuretics Hydrochlorothiazide Loop diuretics Furosemide Potassium -sparing diuretics Spirolactone Diuretics

9. Anesthetics  Animal experiments  Surgery  Pentobarbital sodium and chloral hydrate

10. Procedures  Urine collection:  At therapeutic doses  Self-control ： before and after diuretics or anesthetics oral administration/intraperitoneal injection  LC-MS/MS identification (Triple-TOF 5600)  Label-free quantification  P ˂ 0.05  Fold change ≥2  Spectral count ≥5

11. Results----diuretics AccessionProtein nameFold change Biomarkers P97580beta-microseminoprotein4.7↑ No O35568 EGF-containing fibulin-like extracellular matrix protein 13.5 No Table 3 Urinary proteins significantly changed after hydrochlorothiazide administration

12. Results----diuretics Human orthologs

13. Results---anesthetics

15. Western blot Company Logo

16. Results---anesthetics Human orthologs

17. Summary 1 2 3

18. Acknowledgement  Prof. Youhe Gao  Mindi Zhao  Other students in the laboratory