1. Essentials of Human Anatomy & Physiology Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Slides 10.1 – 10.31 Seventh Edition Elaine N. Marieb Chapter 10 Blood Lecture Slides in PowerPoint by Jerry L. Cook

2. Blood Slide 10.1a Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings  The only fluid tissue in the human body  Classified as a connective tissue  Living cells = formed elements  Non-living matrix = plasma

3. Adults have ~5 Liters of blood.

4. Physical Characteristics of Blood  Color range  Oxygen-rich blood is scarlet red  Oxygen-poor blood is dull red  pH must remain between 7.35–7.45  Blood temperature is slightly higher than body temperature

5. Blood Plasma Slide 10.3  Composed of approximately 90 percent water  Includes many dissolved substances  Nutrients  Salts (metal ions)  Respiratory gases  Hormones  Proteins  Waste products

6. Plasma Proteins Slide 10.4  Albumin – regulates osmotic pressure  Clotting proteins - help to stem blood loss when a blood vessel is injured  Antibodies - help protect the body from antigens

7. Formed Elements  Erythrocytes = red blood cells  Leukocytes = white blood cells  Platelets = cell fragments

10. Erythrocytes (Red Blood Cells)  The main function is to carry oxygen  Anatomy of circulating erythrocytes  Biconcave disks  Essentially bags of hemoglobin  Anucleate (no nucleus)  Contain very few organelles  Outnumber white blood cells 1000:1

11. Hemoglobin  Iron-containing protein  Binds strongly, but reversibly, to oxygen  Each hemoglobin molecule has four oxygen binding sites  Each erythrocyte has 250 million hemoglobin molecules

13. Sickle Cell Anemia A substitution mutation of a single base in the gene for the protein hemoglobin replaces glutamic acid with valine. As a result red blood cells sickle and clog small blood vessels.

14. Sickle Cell Anemia Sickle cell anemia occurs chiefly in black people who live in the malaria belt in Africa and their descendants. Anopheles mosquito carries the malaria parasite.

15. Blood Type

16. ABO Blood Groups

17. Link to Wikipedia prevalence chart

18. Agglutination

19. Rh factor

21. Whole Blood Transfusions Plasma Transfusions

22. Leukocytes (White Blood Cells)  Crucial in the body’s defense against disease  These are complete cells, with a nucleus and organelles  Able to move into and out of blood vessels (diapedesis)  Can move by ameboid motion  Can respond to chemicals released by damaged tissues

23. Leukocyte Levels in the Blood  Normal levels are between 4,000 and 11,000 cells per millimeter  Abnormal leukocyte levels  Leukocytosis  Above 11,000 leukocytes/ml  Generally indicates an infection  Leukopenia  Abnormally low leukocyte level  Commonly caused by certain drugs

24. Types of Leukocytes  Granulocytes  Granules in their cytoplasm can be stained  Include neutrophils, eosinophils, and basophils

25. Types of Leukocytes  Agranulocytes  Lack visible cytoplasmic granules  Include lymphocytes and monocytes

26. Granulocytes  Neutrophils  Multilobed nucleus with fine granules  Act as phagocytes at active sites of infection  Eosinophils  Large brick-red cytoplasmic granules  Found in repsonse to allergies and parasitic worms

27. Granulocytes  Basophils  Have histamine-containing granules  Initiate inflammation

28. Agranulocytes  Lymphocytes  Nucleus fills most of the cell  Play an important role in the immune response  Monocytes  Largest of the white blood cells  Function as macrophages  Important in fighting chronic infection

30. Platelets  Derived from ruptured multinucleate cells (megakaryocytes)  Needed for the clotting process  Normal platelet count = 300,000/mm 3

31. Hemophilia  Inherited disorder that results in diminished clotting ability  Even small cuts can be life-threatening; plasma transfusions are necessary  Genes involved in blood clotting are on the X-chromosome  Occurs more frequently among boys

32. Hematopoiesis  Blood cell formation  Occurs in red bone marrow  All blood cells are derived from a common stem cell (hemocytoblast)  Hemocytoblast differentiation  Lymphoid stem cell produces lymphocytes  Myeloid stem cell produces other formed elements

35. Fate of Erythrocytes  Unable to divide, grow, or synthesize proteins  Wear out in 100 to 120 days  When worn out, are eliminated by phagocytes in the spleen or liver  Lost cells are replaced by division of hemocytoblasts

36. Control of Erythrocyte Production  Rate is controlled by a hormone (erythropoietin)  Kidneys produce most erythropoietin as a response to reduced oxygen levels in the blood  Homeostasis is maintained by negative feedback from blood oxygen levels

37. Control of Erythrocyte Production Figure 10.5

38. Control of Bleeding  Loss of too much blood leads to hypoperfusion, AKA shock. The signs of Shock: 1.Altered mental status (e.g. anxiety, restlessness, combativeness) 2.Pale, cool, clammy skin 3.Nausea and vomiting 4.Vital signs change a.Pulse increases, becoming weak and thready b.Respirations increase, becoming shallow and labored c.Blood pressure drops d.Other signs include thirst, dilated pupils, and cyanosis

39. Control of Bleeding  To Stop Bleeding: 1.Apply direct pressure to the site of bleeding. 2.Elevate the wound above the level of the heart (if possible) 3.Pressure points: Arms=brachial artery, Legs=femoral artery 4.Cold application (in conjunction with other techniques) 5.Tourniquet (this is a last resort)

40. Control of Bleeding  Hemostasis – phases are completed within 3-6 minutes after vessels are broken. 1.Platelet plug formation – platelets, normally repelled by endothelium, become sticky and cling to exposed collagen fibers. A platelet plug (AKA white thrombus) forms 2.Vascular spasms – anchored platelets release serotonin, which causes the tunica media in blood vessels to spasm, decreasing blood flow locally. 3.Coagulation – (a) injured tissue releases thromboplastin, (b) a phospholipid on the surface of platelets, PF 3, interacts with thromboplastin initiating the clotting cascade. 4.Fibrin precipitates – an enzyme in the blood plasma, called thrombin (converted from prothrombin), joins soluble fibrinogen into long fibrin molecules.

41. Hemostasis Link to Hemostasis animation