2. AP Biology 2006-2007 Cellular Respiration Harvesting Chemical Energy ATP

3. AP Biology 2006-2007 What’s the point? The point is to make ATP ! ATP

4. AP Biology Harvesting stored energy  Energy is stored in organic molecules  carbohydrates, fats, proteins  Heterotrophs eat these organic molecules  food  digest organic molecules to get…  raw materials for synthesis  fuels for energy  controlled release of energy  “burning” fuels in a series of step-by-step enzyme-controlled reactions

5. AP Biology Harvesting stored energy  Glucose is the model  catabolism of glucose to produce ATP C 6 H 12 O 6 6O 2 ATP6H 2 O6CO 2  + ++ CO 2 + H 2 O + heat fuel (carbohydrates) COMBUSTION = making a lot of heat energy by burning fuels in one step RESPIRATION = making ATP (& some heat) by burning fuels in many small steps CO 2 + H 2 O + ATP (+ heat) ATP glucose glucose + oxygen  energy + water + carbon dioxide respiration O2O2 O2O2 + heat enzymes ATP

6. AP Biology How do we harvest energy from fuels?  Digest large molecules into smaller ones  break bonds & move electrons from one molecule to another  as electrons move they “carry energy” with them  that energy is stored in another bond, released as heat or harvested to make ATP e-e- ++ e-e- +– loses e-gains e-oxidizedreduced oxidationreduction redox e-e-

7. AP Biology How do we move electrons in biology?  Moving electrons in living systems  electrons cannot move alone in cells  electrons move as part of H atom  move H = move electrons p e + H + H +– loses e-gains e-oxidizedreduced oxidationreduction C 6 H 12 O 6 6O 2 6CO 2 6H 2 OATP  +++ oxidation reduction H e-e-

8. AP Biology Oxidation & reduction  Oxidation  removing H  loss of electrons  releases energy  exergonic  Reduction  adding H  gain of electrons  stores energy  endergonic C 6 H 12 O 6 6O 2 6CO 2 6H 2 OATP  +++ oxidation reduction

9. AP Biology Moving electrons in respiration  Electron carriers move electrons by shuttling H atoms around  NAD +  NADH (reduced)  FAD +2  FADH 2 (reduced) + H reduction oxidation P O–O– O–O– O –O–O P O–O– O–O– O –O–O C C O NH 2 N+N+ H adenine ribose sugar phosphates NAD + nicotinamide Vitamin B3 niacin P O–O– O–O– O –O–O P O–O– O–O– O –O–O C C O NH 2 N+N+ H NADH carries electrons as a reduced molecule How efficient! Build once, use many ways H

10. AP Biology Overview of cellular respiration  3 metabolic stages  Anaerobic respiration 1. Glycolysis  respiration without O 2  in cytosol  Aerobic respiration  respiration using O 2  in mitochondria 2. Krebs cycle 3. Electron transport chain and chemiosmosis C 6 H 12 O 6 6O 2 ATP6H 2 O6CO 2  +++ (+ heat )

11. AP Biology Glycolysis glucose      pyruvate 2x2x 6C3C In the cytosol? Why does that make evolutionary sense? That’s not enough ATP for me!  Breaking down glucose  “glyco – lysis” (splitting sugar)  ancient pathway which harvests energy  where energy transfer first evolved  transfer energy from organic molecules to ATP  still is starting point for ALL cellular respiration  but it’s inefficient  generate only 2 ATP for every 1 glucose  occurs in cytosol

12. AP Biology Evolutionary perspective  Prokaryotes  first cells had no organelles  Anaerobic atmosphere  life on Earth first evolved without free oxygen (O 2 ) in atmosphere  energy had to be captured from organic molecules in absence of O 2  Prokaryotes that evolved glycolysis are (?) ancestors of all modern life  ALL cells still utilize glycolysis

13. AP Biology Pyruvate is a branching point Pyruvate O2O2 O2O2 mitochondria Krebs cycle aerobic respiration fermentation anaerobic respiration

14. AP Biology Fermentation (anaerobic)  Bacteria, yeast 1C 3C2C pyruvate  ethanol + CO 2  Animals, some fungi pyruvate  lactic acid 3C  beer, wine, bread  anaerobic exercise (no O 2 ) NADHNAD + NADHNAD + back to glycolysis 

15. AP Biology  Krebs cycle produces large quantities of electron carriers  NADH  FADH 2  go to Electron Transport Chain! Electron Carriers = Hydrogen Carriers Glucose is completely Oxidized to CO2 H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ ATP ADP + P i

16. AP Biology Electron Transport Chain intermembrane space mitochondrial matrix inner mitochondrial membrane NAD + Q C NADH H 2 O H+H+ e–e– 2H + +O2O2 H+H+ H+H+ e–e– FADH 2 1212 NADH dehydrogenase cytochrome bc complex cytochrome c oxidase complex FAD e–e– H H  e- + H + NADH  NAD + + H H p e Building proton gradient! Creates a proton gradient!

17. AP Biology  ATP synthase enzyme  H + flows through it  conformational changes  bond P i to ADP to make ATP  The ETC’s H + gradient  allows the H + to flow down concentration gradient through ATP synthase  ADP + P i  ATP H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ H+H+ ATP ADP P + And how do we make ATP?

18. AP Biology Summary of cellular respiration  Where did the glucose come from?  Where did the O 2 come from?  Where did the CO 2 come from?  Where did the CO 2 go?  Where did the H 2 O come from?  Where did the ATP come from?  What else is produced that is not listed in this equation?  Why do we breathe? C 6 H 12 O 6 6O 2 6CO 2 6H 2 O~40 ATP  +++