1. Occur in the Glycolytic Pathway
Four Important Events
Occur in the Glycolytic Pathway
Substrate level phosphorylation: the transfer of phosphate groups from substrates to ATP
Breaking of a six-carbon molecule (glucose) into two three-carbon molecules of pyruvate
The reduction of two coenzymes of NAD to NADH
The capture of energy in ATP 1

2. Fermentation Pyruvic Acid Types of fermentation
metabolized in the absence of oxygen
NAD must be recycled
NAD passes electrons to other molecules
Types of fermentation
Lactic acid
Pyruvic acid converted to lactic acid
uses electrons from NAD
Alcoholic
carbon dioxide released from pyruvic acid
Acetaldehyde formed
reduced to ethanol 2

3. Lactic Acid Fermentation: Pyruvic acid reduced to lactic acid by NAD from glycolysis3

4. Alcoholic Fermentation: Carbon dioxide removed from pyruvic acid to acetaldehyde; Acetaldehyde reduced to ethyl alcohol by NAD 4

5. Results of Glucose Fermentation
Natural waste products useful to humans
Fermented beverages
Bread
Cheese
Yogurt
Infectious microbes may cause disease 5

6. Fermentation Pathways6

7. Anaerobic Respiration
Starts with glycolysis
Yields ATP’s
Complete oxidation of substrate molecules to C02 in the Krebs Cycle
Glycolysis yields 2 ATP’s net
Partial oxidation of carbon atoms 7

8. Transition to the Krebs cycle: Pyruvic acid loses CO2 & gets oxidized by NAD; two-carbon acetyl group attaches to coenzyme A, forming acetyl-CoA. 8

9. The Reactions of the Krebs Cycle9

10. 10

11. 11

12. 12

13. 13

14. 14

15. 15

16. 16

17. What has been accomplished in the Krebs Cycle?
8 reactions instead of just 1
Each reaction has a separate reactant-specific enzyme
The initial reactants are two 2-carbon molecules of acetyl Co-A and the final products are four 1-carbon molecules of CO2 .
Transfer of electrons and/or H+ to coenzymes – 3 pairs to NAD and 1 to FAD for each turn of the cycle
One ATP molecule produced for each turn of the cycle
The cycle turns two times for each molecule of glucose because glucose has been converted to two molecules of acetyl Co-A
The cycle regenerates the starting molecule, oxaloacetate 17

18. Why is Krebs a Cycle?
Oxaloacetic acid combines with acetyl-CoA in the first step
Oxaloacetic acid is regenerated at the end 18

19. What happens to the Coenzymes?
They must be reoxidized!
Their chemical bonds have stored chemical energy.
Coenzymes are in short supply.
Must be available to continue their oxidation work in the Krebs Cycle 19

22. Waterfall model of the electron transport chain
As electrons pass from carrier to carrier, they decrease in energy.
Some of the energy they lose is harnessed to make ATP. 22

23. The Electron Transport Chain
The electron transport chain performs two functions:
Accepts electrons from NADH and FADH2 & transfers them to electron acceptors
Uses the energy released during the electron transfers to pump H+ across the inner mitochondrial membrane to the intermembrane space creating a concentration gradient 23

24. Enzyme Complexes Involved in Electron Transport
Oxidation/reduction enzymes:
1. NADH dehydrogenase
2. Flavoproteins (FAD)
3. Iron-sulfur proteins
4. Cytochromes
5. Quinones (lipid-soluble) 24

27. Electron Transport Chain
Transfers electrons from one substrate to another and finally to oxygen
Pumps H+ across the inner mitochondrial membrane into the inter-membrane space
Oxygen is the final electron and hydrogen acceptor! 27

28. The Electron Transport Chain28

29. Chemiosmosis
The electron transport chain creates a H+ gradient potential across the inner mitochondrial membrane
ATP Synthase allows H+ back across the membrane
ATP is produced by a proton motive force (pmf) when H+ pass through ATP Synthase and cause ADP + P to form ATP. 29

32. Oxidative Phosphorylation
ATP formation involving molecular oxygen and chemiosmosis

35. What part of the electron transport chain is represented by the marbles being pushed upward?
The hydrogens being pumped into the inter-membrane space

36. How does electronegativity play a part in the electron transport chain?
Each electron acceptor in the chain is more electronegative than the previous one
the electrons move from one electron transport chain molecule to the next, falling closer and closer to the nucleus of the last electron acceptor.

37. Where do the electrons for the electron transport chain come from?
NADH and FADH2 which got their electrons from glucose originally, in the previous two phases of cell respiration.

38. Why does FADH2 drop its electron onto a different initial acceptor than NADH?
It has a different electronegativity.
FADH2 is more electronegative and therefore the initial acceptor for FADH2 must be stronger in order to pull the electrons away.

39. What molecule is the final electron acceptor?
Water is made from the splitting of an oxygen gas molecule. Each oxygen atom grabs
two electrons from the electron transport chain and
two hydrogen ions from the inter-membrane space.
What is consumed during this process?
Oxygen gas

40. What is gained by this process?
A chemiosmotic gradient of H+ ions inside the inter-membrane space.

41. The electron transport chain does not generate ATP directly, so what good does it do?
It generates a chemiosmotic gradient that will eventually generate ATP.

42. How does this gradient generate ATP?
A special protein, ATP Synthase, embedded in the inner membrane can use the flow of hydrogen ions, H+, to phosphorylate ADP molecules.

43. How does the specialized membrane protein work?
It turns mechanically like a rotary motor
As the hydrogen ions, H+, rush through the protein channel, it causes chemical energy to be converted into mechanical energy
This energy drives a phosphorylation reaction.
This is called oxidative phosphorylation.

44. Is cell respiration endergonic or exergonic?
Exergonic – energy is released.

45. Is it a catabolic or anabolic reaction?
Catabolic – a molecule is being broken down into smaller molecules

46. If one molecule of ATP holds 7
If one molecule of ATP holds 7.3 kcal of potential energy, how much potential energy does one molecule of glucose produce in cell respiration?
At its maximum output:
38 X 7.3 kcal = kcal

47. One molecule of glucose actually contains 686 kcal of potential energy
One molecule of glucose actually contains 686 kcal of potential energy. Where does the remaining energy go when glucose is oxidized?
It is lost as heat which is why we’re warm.

48. What is the net efficiency of cell respiration if glucose contains 686 kcal and only kcal are produced?
277.4 / 686 x 100 = 40%

49. How does this rate of efficiency compare to energy capture processes that you see in everyday life?
Incandescent light bulb = 5% efficient
Electricity from coal = 21% efficient
Car engines = 23% efficient

50. Define cellular respiration.
Cellular respiration is the release of energy from food by oxidation.

51. Compare anaerobic and aerobic respiration.

52. Anaerobic Respiration
Starts with glycolysis
Yields ATP’s
Complete oxidation of substrate molecules to C02 in the Krebs Cycle
Glycolysis yields 2 ATP’s net
Partial oxidation of carbon atoms 52

53. Why doesn’t the reaction stop once the ATP’s are produced from glycolysis leaving the oxidized glucose in the form of pyruvate?
Because the reactions that produce CO2 + alcohol or lactic acid are needed to reoxidize NADH. Without this the lack of NAD+ would stop glycolysis.

54. Given what you know about the process of fermentation, what are some of the requirements for making wine or beer?
A culture of yeast or other anaerobic organisms
An oxygen-free environment so the organisms are forced to perform only glycolysis
A source of glucose or fructose

55. Total Energy Released
“The oxidation of glucose to carbon dioxide releases approximately kcal of energy. If all of this energy is released at one time, then most of it would be lost as heat. Burning the energy all at once would be akin to igniting your gas tank in order to run your car, rather than burning small amounts of gasoline slowly in the engine. If the energy of glucose is released slowly, in several small steps, then the potential energy available could be captured at each small step, just as the gasoline energy is captured (to move a car) in a slow release rather than in a single, explosive release.”