1. Humoral and Cellular Immunity
Kaynak: Abbas 2003, Male 2006, Le Bien Blood 2008
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2. Presentation Plan Basics of immune response Lymphocyte types
Primary and secondary lymphoid tissues
Development of B lymphocyte
B lymphocyte functions
Development of T lymphocytes
Receptors of lymphocytes and activation
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3. Objectives of this lecture
You can tell:
Development of B lymphocytes
Functions of B lymphocytes
Development of T lymphocytes
Functions of T lymphocytes
You can explain:
Humoral immunity
Cellular immunity
Differencies between both of them
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4. Dr. Yıldıran

5. Specific immunity (Adapttive) Non-specific immunity (Innate)
Immune System
Specific immunity (Adapttive)
Non-specific immunity (Innate)
Phagocytes
NK cells
Complement system
T and B lymphocytes
MEMORY!
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6. Pathogen Immune System Extracellular Intracellular Toxin Microbiome
Lactobacillus
Flora
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7. Antigen presenting cell
Immune Response
Antigen presenting cell
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Abbas, 2003

8. Adaptive immunity
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9. Specific Immunity
CD19
CD3
CD4
CD3CD8
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Abbas, 2003

10. Maturation of Lymphocytes
B lymphocytes in bone marrow
T lymphocytes in thymus
Then they include circulation as naive lymphocytes
IL-7 is essential in this process.
If naive lymphocytes don’t encounter to any foreign antigen they go to apopitosis (programmed death).
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11. Antigens are catched and processed here
Primary Lymphoid Tissues
Thymus  Development of T lymphocytes
Fetal liver and postnatal bone marrow  Development of B lymphocytes
Secondary Lymphoid Tissues
Spleen
Lymph nodes
MALT (mucosa associated lymphoid tissue)
Antigens are catched and processed here
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12. B lymphocyte development
Early Pre-B cell
LAte Pre-B cell
Stem cell
Pro-B cell
Pre-BCR
Fetal
Liver
Antigen independent
Bone marrow
Immatüre B cell
Mature B cell
Plasma cell
Antigen dependent

13. Markers
CD10+
Conley 2003

14. Genetic defects of B cell development

15. Class (isotype) switching (CSR): B cell produce firstly IgM, then switch this molecule to other Ig’s.
Somatic hypermutation involves a programmed process of mutation affecting the variable regions of immunglobulin genes  immune repertoire
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16. Antigen specific receptor
Somatic recombination provides this with RAG (Recombination activating gene) ensyme
If this gene disrupted, the result is severe combined immunodeficiency (SCID).
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17. Immunglobulin Molecule
Antigen binding site (Fab)
Fc receptor and complement binding site
Disulphit links
Carbohydrate
Heavy chain
Light chain
Ig domains
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18. Functions of antibodies Complement activation
Neutrolastion of microorganisms and toxins
Antibodies
B lymph
Opsonisation and phagoctosis
Microorganism
Lysis
Phagocytosis of opsonised microorganism by complement
Complement activation
Inflammation
IgG
Surface antigen
FcRIII
Antibody dependent cellular cytotoxicity
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NK cell

19. Maturation of T lymphocyte
CD4+ T hücresi
(Thelper=Th)
CD8+ T cell
Cytotoxic T cell
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20. Selection of T lymphocyteDP DN
Zone 1-4 SP
AIRE
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21. Receptors
CTLA-4=CD152
Inhibitor
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22. Activation
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23. Activated Lymphocytes
Activation markers
CD25 and CD69
Effector molecules
CD40L (=CD154) activates B lymphocytes, dendritic cells, endothelial cells
Fas (CD95)  apopitosis
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24. Peptide
Antigen catch
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25. Antigen processing CD4  IL-2, IFNG CD8  Perforin, granzym Antigen
Presentig Cell
ER: Endoplasmic Reticulum
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26. Signaling
IL-2, IL-4,
IL-5, IFN secreted
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27. Functions of T Lymphocytes
Defense to intracellular microorganisms
Th cytokine secretion, stimulation of macrophages
Activation of B lymphocytes and phagocytes Th
Killing of infected cells Ts
Memory
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28. memory T
TCR specifity and diversity, recognize different antigens.

29. Flowcytometry